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Device and method for culturing and detecting hydrotropism of root of Arabidopsis thaliana

A technology of Arabidopsis thaliana roots and Arabidopsis thaliana, which is applied in the field of devices for cultivating and detecting Arabidopsis root hydrotropism, and can solve the problems of lack of reports and other issues

Pending Publication Date: 2019-04-30
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to design a device and method for cultivating and detecting plant root hydrotropism after constructing a water potential gradient under different light conditions is still lacking in reports

Method used

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  • Device and method for culturing and detecting hydrotropism of root of Arabidopsis thaliana
  • Device and method for culturing and detecting hydrotropism of root of Arabidopsis thaliana
  • Device and method for culturing and detecting hydrotropism of root of Arabidopsis thaliana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 Or as shown in 2, the device for cultivating and detecting the water-tropism of Arabidopsis roots includes a box body 1 with an open top, and the top of the box body 1 is sequentially provided with light-transmitting panels that are slidably connected to the box body 1 from bottom to top 2 and shading plate A 3, at least one side wall of the box body 1 is formed by a transparent material, and the side wall formed by the transparent material is covered with a light shading plate B 4 that is slidably connected with the box body 1, and the inside of the box body 1 A fixing piece is provided on the wall, and the fixing piece is used for fixing the petri dish 5 on which the Arabidopsis seedlings 15 are bonded, and a light source 6 is arranged in the upper part of the box body 1 .

[0032] Both sides of the opening are sequentially provided with a first chute 7 and a second chute 8 from bottom to top, the light-transmitting plate 2 is slidably connected with ...

Embodiment approach

[0033] As an embodiment of the present invention, the fixture includes a support block 10 and a positioning block 11, the support block 10 is arranged at the lower part of the inner side wall of the box body 1 and is used to support the bottom of the culture dish 5, and the positioning block 11 is arranged at the support block 10 above and used to clamp and fix the 5 middle parts of the petri dish.

[0034] In this embodiment, the support block 10 is two triangular blocks arranged at intervals, and the bottom ends of the culture dish 5 placed vertically and bonded with Arabidopsis seedlings are fixed against the two triangular blocks respectively.

[0035] The box body 1 is provided with a thermometer 12 and a hygrometer 13, which can dynamically detect changes in temperature and humidity in real time, and a timer 14 is provided on the outer wall 4 of the shading plate B for convenient timing. The bottom of the box body 1 is provided with a saturated saline solution, and the l...

Embodiment 2

[0039]The method for cultivating and detecting Arabidopsis root water-tropism using the device of the present invention comprises the following steps:

[0040] 1) Pour the sterilized 1 / 2 MS medium (1% agar) into a petri dish, cut off 2 / 3 of the lower part of the medium (1% of the upper part of the medium) neatly with a sterile scalpel after cooling. / 3 for experiments), and then move the Arabidopsis seedlings grown for 5-7 days to the surface of 1 / 3 of the medium after removal so that the Arabidopsis seedlings stick to the surface of the medium along the seedling height direction, and make the Arabidopsis 2-3mm from the root tip of Arabidopsis thaliana is exposed to the edge of the medium;

[0041] 2) Add saturated salt solution (saturated K 2 CO 3 solution or saturated CaCl 2 solution), the liquid level of the saturated saline solution is 1 / 10-1 / 5 of the height of the box, and the culture dish bonded with Arabidopsis seedlings is placed vertically on the inner wall of the ...

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Abstract

The invention discloses a device and a method for culturing and detecting hydrotropism of a root of Arabidopsis thaliana. The device includes a box body having an opening at the top; the top of the box body is sequentially from bottom to top provided with a light-transmitting plate and a light-shielding plate A which are slidably connected to the box body; at least one side wall of the box body isformed of a transparent material; the outside of the side wall formed by the transparent material is covered with a light shielding plate B slidably connected to the box body; the inner side wall ofthe box body is provided with a fixing member; the fixing member is used for bonding a culture dish having seedlings of the Arabidopsis thaliana; and a light source is disposed on the upper portion ofthe inside of the box body. The device and the method utilize a humidity gradient environment designed by agar-air coupling, and so, the hydrotropism of the root of the Arabidopsis thaliana can be simulated, operation is flexible and convenient, and the method is effective and stable.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a device and method for cultivating and detecting Arabidopsis root hydrotropism. Background technique [0002] The cultivation test is a plant growth test conducted under artificial simulation or artificial control conditions in facilities such as greenhouses, net rooms or artificial climate chambers, where the growth medium is placed in a special container. Plant roots are the main organ for absorbing water and nutrients, and also an important site for the assimilation and synthesis of various hormones, organic acids and metabolites. The hydrotropism of roots is the characteristic that roots tend to grow in wetter places when the water distribution in the soil is uneven. However, there is still a lack of reports on how to design an experimental device that is closer to the soil moisture gradient in the natural environment to study hydrotropism. [0003] There are few ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/02A01G7/04
CPCA01G7/045A01G31/02Y02P60/21
Inventor 许卫锋李瑛李落成萧蔚袁伟
Owner FUJIAN AGRI & FORESTRY UNIV
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