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Enterococcus faecium DT1-1 and application thereof

A technology for Enterococcus faecium and microbial strains, which can be used in applications, bacteria, microorganisms, etc., and can solve the problems of poor tolerance, small variety and quantity, etc.

Active Publication Date: 2019-04-26
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most probiotics have poor tolerance to the high temperature, transportation, gastrointestinal fluid and bile of the granulation process in feed processing, and the types and quantities of live lactic acid bacteria entering the intestinal tract are not many. Probiotics in intestinal juice and bile are the key to solving this series of problems

Method used

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  • Enterococcus faecium DT1-1 and application thereof
  • Enterococcus faecium DT1-1 and application thereof
  • Enterococcus faecium DT1-1 and application thereof

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Experimental program
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Embodiment Construction

[0017] The present invention will be explained in detail below.

[0018] 1. Isolation of strains

[0019] The Enterococcus faecium DT1-1 (Enterococcus faecium DT1-1) of the present invention is isolated from healthy yak feces in Qinghai. The bacterial strain separation and purification method of the present invention is: take by weighing 10g of yak feces, after diluting and mixing with 100mL TS Broth and filtering, take 1mL of the mixed liquid and dilute respectively for 10 -4 , 10 -5 , 10 -6 For the next step, 0.1 mL each was spread on a calcium-containing MRS plate, and after the dilution was absorbed by the plate, cultured anaerobically at 37°C for 24 hours. Selected 32 single colonies suspected of lactic acid bacteria with calcium-dissolving circles, respectively streaked and purified on the MRS solid plate for 3 times, and 12 strains were identified as Enterococcus faecium by 16S molecular identification, and each single colony was selected on the MRS Static cultivati...

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Abstract

The invention discloses a probiotic, enterococcus faecium DT1-1 and an application thereof. The strain provided by the invention was preserved in the China General Microbiological Culture Collection Center on October 22, 2018, and the preservation number of the strain is CGMCC No.16603. The enterococcus faecium DT1-1 provided by the invention has strong adhesion and good bacteriostasis, good acidresistance, gastrointestinal resistance and bile salt resistance, and can be used in feeding herbivorous animals, or preparing feed additives for herbivorous animals, or preparing feed for herbivorousanimals.

Description

technical field [0001] The invention relates to a strain of Enterococcus faecium DT1-1 and its application. Background technique [0002] Since the 20th century, while the addition of antibiotics in feed has made a significant contribution to the development of intensive animal husbandry, its side effects such as endogenous infection and superinfection, the emergence of drug resistance, the destruction of normal intestinal flora and the Residues in products and the environment pose a serious threat to aquaculture, animal and human food safety, and have also attracted people's attention. Probiotic preparations are mainly live microorganisms. After the host ingests a certain amount, a certain number of strains can adhere, colonize and grow in specific parts of the gastrointestinal tract. The preparation that plays a beneficial role in animal growth and health is a new green and safe additive that is a good substitute for antibiotics. [0003] However, most probiotics have po...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCA23K10/18C12R2001/01C12N1/205
Inventor 李有全李禤王锦明刘军龙刘爱红关贵全贾丹殷宏罗建勋刘志杰独军政高闪电刘光远任巧云陈泽杨吉飞
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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