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Controlled release system based on base pairing rules as well as preparation method and application thereof

A base-pairing and regular technology, applied in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., to achieve controllable drug release operation, excellent photothermal conversion effect, and activation of anti-tumor immunity. Effect

Active Publication Date: 2019-04-26
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the deficiencies in the prior art and provide a controlled-release system DOX&ICG@MSN-T@polyA for assembling complex gating systems based on base pairing rules and its preparation method and application

Method used

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  • Controlled release system based on base pairing rules as well as preparation method and application thereof
  • Controlled release system based on base pairing rules as well as preparation method and application thereof
  • Controlled release system based on base pairing rules as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 According to figure 1 As shown, the preparation method of DOX&ICG@MSN-T@polyA controlled release system based on base pairing includes the following steps:

[0051] The first step is MSN preparation. Dissolve 0.5g CTAB in 240mL distilled water with magnetic stirring, add 1.75mL NaOH, heat to 80°C to obtain a CTAB / NaOH mixture; then add 2.5mLTEOS dropwise, continue stirring for 2 hours, centrifuge to collect the precipitate, wash with distilled water and methanol to obtain MSN Crude product: 0.7g of MSN obtained was dispersed in 70mL methanol and 0.70mL, 37.2wt% concentrated HCl mixture, refluxed for 12 hours to remove the template agent CTAB, and the solid obtained after centrifugation was washed 6 times with distilled water to obtain purified MSN.

[0052] The second step is the preparation of thymine-modified MSN (MSN-T). Mix 0.5 g of purified MSN and 0.5 mL of ATPES in 50 mL of anhydrous toluene, N 2 After reflux at 80°C for 24 hours under atmosphere, c...

Embodiment 2

[0054] Embodiment 2: In order to better understand the performance of the target substance obtained in this embodiment 1, it is characterized by the following tests:

[0055] 1. Morphology test of DOX&ICG@MSN-T@polyA controlled release system

[0056] The specific test steps are: wash DOX&ICG@MSN-T@poly A with distilled water several times, ultrasonically for 10 minutes, then drop it on the copper grid, wait for its moisture to dry naturally, and test it on the JOEL JEM-2100F high-resolution transmission electron microscope Operating at an accelerating voltage of 200 kV, high-resolution TEM (HRTEM) images were taken.

[0057] Such as figure 2 As shown, the size, shape and pore blocking of the nanoparticles were observed by transmission electron microscopy. The results showed that the nanoparticles had regular shapes, well-dispersed, uniform sizes, the pores were blocked, and the edges became blurred, indicating that the drug was successfully loaded and the surface was succes...

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Abstract

The invention discloses a controlled release system based on base pairing rules as well as a preparation method and application thereof. The controlled release system is prepared by encapsulating mesoporous silica nanoparticles (MSN) modified by thymine used as a carrier, and chemotherapy drugs DOX (Doxorubicin Hydrochloride) and thermotherapy drugs ICG (Indocyanine Green), which are loaded in pore channels of the nanoparticles, in polyadenylic acid through the base pairing rules. The mesoporous silica nano-material has the advantages of ultra-large mesoporous volume, high specific surface area, relatively good biocompatibility, easiness for surface modification and the like, can be used for encapsulating a lot of drugs and is a good carrier platform; the polyadenylic acid is used as a gating blocking matter and can be used for effectively blocking mesoporous and controlling the release of the drugs, so that an A-T hydrogen bond is triggered to be cracked after the controlled release system is irradiated through near-infrared light and the drugs DOX and ICG are released; the drug release is controlled better and the chemotherapy and optical thermotherapy cooperative treatment is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a controlled-release system based on base pairing rules, a preparation method thereof and an application in the field of combined tumor therapy. Background technique [0002] For tumor treatment, more significant therapeutic effects can often be obtained through the development of multimodal treatment, such as the combination of chemotherapy and hyperthermia. The main reasons are as follows: high temperature will affect the function of tumor cell plasma membrane, increase membrane permeability, which is beneficial to drug uptake; many drugs have heat-sensitizing properties, which can increase drug toxicity; when applied in vivo tumor treatment, the central part of tumor tissue is easily heated and damaged. Reach a higher temperature, but there are more blood vessels in the surrounding parts, the heat diffusion is faster, and the effect of hyperthermia is not obvio...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K41/00A61K31/704A61K47/54A61P35/00
CPCA61K41/0052A61K47/549A61K47/6949A61P35/00A61K9/0019A61K31/704A61K2300/00
Inventor 周家宏周林李晓婷魏少华
Owner NANJING NORMAL UNIVERSITY
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