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Lipid antibacterial composition, extraction method, detection method and use

The technology of an antibacterial composition and a detection method, which is applied in the biological field, can solve problems such as difficulty in obtaining antibacterial effects, and achieve the effects of good extraction effect and high extraction rate

Active Publication Date: 2021-02-02
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 2. Foreign literature reports have confirmed that reasonable immune induction can make black soldier fly larvae produce antibacterial peptides and exhibit antibacterial effects. Correspondingly, without immune induction, it is difficult to obtain satisfactory antibacterial effects

Method used

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  • Lipid antibacterial composition, extraction method, detection method and use
  • Lipid antibacterial composition, extraction method, detection method and use
  • Lipid antibacterial composition, extraction method, detection method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The separation and preparation steps of the 3rd instar black soldier fly larva lipid antibacterial extract are as follows:

[0031] (1) Empty the intestinal contents of black soldier fly larvae

[0032] The 3rd instar black soldier fly larvae were placed in the breeding box, and they were not fed overnight until the intestinal contents of the larvae were cleared.

[0033] (2) Cleaning and disinfection

[0034] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.

[0035] (3) Removal of larval gut

[0036] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at...

Embodiment 2

[0044] The separation and preparation steps of the lipid antibacterial extract of 3.5 instar black soldier fly larvae are as follows:

[0045] (1) Empty the intestinal contents of black soldier fly larvae

[0046] The 3.5-instar black soldier fly larvae were placed in the breeding box, and were not fed overnight until the intestinal contents of the larvae were cleared.

[0047] (2) Cleaning and disinfection

[0048] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.

[0049](3) Removal of larval gut

[0050] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at l...

Embodiment 3

[0056] The separation and preparation steps of the 4th instar black soldier fly larva lipid antibacterial extract are as follows:

[0057] (1) Empty the intestinal contents of black soldier fly larvae

[0058] The 4th instar black soldier fly larvae were placed in the breeding box, and they were not fed overnight until the intestinal contents of the larvae were cleared.

[0059] (2) Cleaning and disinfection

[0060] Put the black soldier fly larvae that had been fasting overnight into a conical flask, wash it three times with distilled water, filter out the water, sterilize it once with 75% ethanol, and filter it out for later use.

[0061] (3) Removal of larval gut

[0062] The spare larvae were filtered and placed on an ultra-clean bench (SW-CJ-IF), operated aseptically, carefully incised with a scalpel along the midline of the larvae's abdomen, and the larvae intestines were taken out with sterile tweezers. The parasites and intestines were stored in clean containers at...

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Abstract

The invention discloses a lipid antibacterial composition. The lipid antibacterial composition is extracted from black soldier fly larvae, and contains oleic acid glyceride 18.0%-22.0%, lauric acid 7.7%-10.0%, myristic acid 15.5% to 18.0%, the present invention belongs to the field of biology, the composition has excellent antibacterial effect, and can at least have a specific antibacterial effect on some Escherichia coli and Salmonella enteritidis, and the present invention also discloses the extraction method of the composition , detection methods and uses.

Description

technical field [0001] The invention relates to the field of biology, in particular to a lipid antibacterial composition, an extraction method, a detection method and application. Background technique [0002] "Research Progress on Black Soldier Fly", Breeding and Feed; No. 12, 2015; author Liu Hongyu et al disclosed the following content: Through the screening of gut microbes of black soldier fly larvae, the obtained strains can synthesize certain Lipopeptide surfactants, such as lipopeptide Iturin (subtilin) ​​and Surfaction (surfaction), and it is speculated that lipopeptide Iturin (subtilin) ​​and Surfaction (surfaction) have certain antibacterial effects, while The article also records that Korean scientists extracted the antibacterial peptides in the black soldier fly by induction, which showed a good antibacterial effect, and emphasized that the antibacterial effect occurred after immune induction. [0003] "Research and Prospects on Characterization of Lipopeptide S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/231A61K31/20A61P31/04G01N30/02G01N30/06
CPCA61K31/20A61K31/231A61P31/04G01N30/02G01N30/06A61K2300/00Y02A50/30
Inventor 容庭张洁刘志昌王刚马现永李书宏邓盾田志梅崔艺燕
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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