A kind of probiotic composition and its application
A composition and technology of probiotics, applied in the field of microorganisms, can solve problems such as the lack of research on probiotic compound formulas, achieve the effects of reducing fasting blood sugar levels, wide application prospects, and reducing epididymal fat content
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[0040] The present invention also provides a preparation method of the probiotic composition, inoculating the liquids of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 into the improved MRS medium respectively, at 35-37°C Cultivate under anaerobic conditions for 18-24 hours, collect the bacteria; resuspend with freeze-drying protectant respectively to make the content of each bacteria reach 10 10 CFU / mL or more, then the suspension was cultured under anaerobic conditions at 37°C for 40-60 minutes, dried to obtain freeze-dried bacterial powder of each bacterial cell, and the number of viable bacteria in the bacterial powder was confirmed by resuspension and dilution plate coating; The freeze-dried bacteria powder of Bifidobacterium adolescentis CCFM8630, the freeze-dried bacteria powder of Lactobacillus reuteri CCFM8631 and the freeze-dried bacteria powder of Lactobacillus rhamnosus CCFM1044 are compounded and mixed in ...
Embodiment 1
[0073] Example 1: Probiotic composition has good tolerance to simulated gastrointestinal fluid
[0074] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 stored at -80°C were inoculated in modified MRS medium (mMRS) respectively, and cultured in an anaerobic workstation at 37°C for 36 hours. Subculture 2 times with the inoculation amount of 2%-4% (v / v), adjust the live bacteria concentration of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution to be 5×10 8 CFU / mL, take the concentration as 5×10 8 CFU / mL of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution 1mL each, mix well, take 1mL of mixed culture solution and 9.0mL pH 2.5 artificial simulated gastric juice (containing 1% stomach Protease, mMRS medium with pH=2.5) mixed, cultured in anaerobic environment at 37°C, samples ...
Embodiment 2
[0081] Example 2: The probiotic composition has no toxic and side effects on C57BL / 6J mice
[0082] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 with a live bacterial count ratio of 1:1:1 were resuspended in 3% sucrose solution to make a concentration of 1.0×10 9 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20g-22g were taken, and after one week of adaptive feeding, they were fed with the bacterial suspension of this concentration once a day, observed for one week, and the death and body weight were recorded. The experimental results are shown in Table 3.
[0083] Table 3 Changes in body weight and death of mice
[0084] time (days) 1 2 3 4 5 6 7 weight (g) 20.2±0.9 20.4±1.0 20.5±1.3 20.7±1.1 20.8±0.8 21.2±0.9 21.6±0.8 Circumstances of death - - - - - - -
[0085] Note: -: no death of mice
[0086] The results showed that ...
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