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A kind of probiotic composition and its application

A composition and technology of probiotics, applied in the field of microorganisms, can solve problems such as the lack of research on probiotic compound formulas, achieve the effects of reducing fasting blood sugar levels, wide application prospects, and reducing epididymal fat content

Active Publication Date: 2021-03-19
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use a single probiotic or a combination of probiotics and prebiotics or Chinese herbal medicines to relieve metabolic syndrome, and currently there are few probiotic compound formulas that can synergistically improve metabolic syndrome. Research

Method used

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  • A kind of probiotic composition and its application
  • A kind of probiotic composition and its application
  • A kind of probiotic composition and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] The present invention also provides a preparation method of the probiotic composition, inoculating the liquids of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 into the improved MRS medium respectively, at 35-37°C Cultivate under anaerobic conditions for 18-24 hours, collect the bacteria; resuspend with freeze-drying protectant respectively to make the content of each bacteria reach 10 10 CFU / mL or more, then the suspension was cultured under anaerobic conditions at 37°C for 40-60 minutes, dried to obtain freeze-dried bacterial powder of each bacterial cell, and the number of viable bacteria in the bacterial powder was confirmed by resuspension and dilution plate coating; The freeze-dried bacteria powder of Bifidobacterium adolescentis CCFM8630, the freeze-dried bacteria powder of Lactobacillus reuteri CCFM8631 and the freeze-dried bacteria powder of Lactobacillus rhamnosus CCFM1044 are compounded and mixed in ...

Embodiment 1

[0073] Example 1: Probiotic composition has good tolerance to simulated gastrointestinal fluid

[0074] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 stored at -80°C were inoculated in modified MRS medium (mMRS) respectively, and cultured in an anaerobic workstation at 37°C for 36 hours. Subculture 2 times with the inoculation amount of 2%-4% (v / v), adjust the live bacteria concentration of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution to be 5×10 8 CFU / mL, take the concentration as 5×10 8 CFU / mL of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 culture solution 1mL each, mix well, take 1mL of mixed culture solution and 9.0mL pH 2.5 artificial simulated gastric juice (containing 1% stomach Protease, mMRS medium with pH=2.5) mixed, cultured in anaerobic environment at 37°C, samples ...

Embodiment 2

[0081] Example 2: The probiotic composition has no toxic and side effects on C57BL / 6J mice

[0082] Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044 with a live bacterial count ratio of 1:1:1 were resuspended in 3% sucrose solution to make a concentration of 1.0×10 9 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20g-22g were taken, and after one week of adaptive feeding, they were fed with the bacterial suspension of this concentration once a day, observed for one week, and the death and body weight were recorded. The experimental results are shown in Table 3.

[0083] Table 3 Changes in body weight and death of mice

[0084] time (days) 1 2 3 4 5 6 7 weight (g) 20.2±0.9 20.4±1.0 20.5±1.3 20.7±1.1 20.8±0.8 21.2±0.9 21.6±0.8 Circumstances of death - - - - - - -

[0085] Note: -: no death of mice

[0086] The results showed that ...

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Abstract

The invention relates to the technical field of microorganisms, and discloses a probiotic composition and its application. The probiotic composition of the present invention is composed of Bifidobacterium adolescentis CCFM8630, Lactobacillus reuteri CCFM8631 and Lactobacillus rhamnosus CCFM1044, the effect of relieving metabolic syndrome is obviously better than CCFM8630 or CCFM8631 used alone or in combination, especially in Decrease in epididymis fat content, decrease fasting blood glucose level, decrease area under the glucose tolerance curve, decrease serum low-density lipoprotein and total cholesterol levels, improve liver antioxidant capacity and decrease serum IL‑1β content, etc. The single-bacteria or double-bacteria combination formula has increased by 9.62%-769.62%, and the three-bacteria combination can achieve significant synergistic effect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a probiotic composition and its application, in particular to a probiotic composition for alleviating metabolic syndrome and its application. Background technique [0002] Metabolic syndrome is a group of diseases closely related to obesity, diabetes, hypertension, and cardiovascular disease, and its clinical manifestations are abnormal glucose tolerance, overweight or obesity, dyslipidemia, and hypertension. Numerous studies have shown that metabolic syndrome is a major contributor to cardiovascular disease, and people with metabolic syndrome are also more likely to develop inflammation and are also prone to other diseases, such as polycystic ovary syndrome, fatty liver, cholesterol gallstones, Asthma, sleep disturbances, and certain cancers. In addition, metabolic syndrome is significantly associated with the prevalence and mortality of coronary atherosclerotic heart di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L33/00A23L11/50A23L11/65A23L19/00A23C9/123A23C13/16A23C19/032A23C19/06A61K35/747A61P3/04A61P3/10A61P3/06A61P29/00A61P39/06A61K35/745C12R1/01C12R1/225A23L11/60
CPCA61P3/04A61P3/06A61P3/10A61P29/00A61P39/06A23C9/1234A23C13/16A23C19/0323A23C19/062A23L19/00A23L29/065A23L33/00A23L33/135A61K35/745A61K35/747C12N1/20A23V2002/00A23L11/50A23V2400/173A23V2400/513A23V2400/175A23V2200/332A23V2200/328A23V2200/3262A23V2200/30A61K2300/00A61P1/00A23L33/175A61K2035/115
Inventor 王园园陈卫王刚朱晓风马方励肖俊勇张灏赵建新
Owner INFINITUS (CHINA) CO LTD
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