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Application of artemisia absinthium extract to preparing anti-hepatoma medicine

An extract and anti-liver cancer technology, applied in drug combinations, anti-tumor drugs, pharmaceutical formulations, etc., can solve problems such as limited treatment methods, response rate of less than 4%, and objective remission of less than 20% of patients

Pending Publication Date: 2019-04-23
XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are limited treatments for advanced liver cancer. Sorafenib is the first-line drug approved by the US FDA for the treatment of advanced liver cancer, but Sorafenib can only prolong the survival of patients by 3 months, and the response rate is less than 4 %
The cancer immunotherapy Keytruda (Pembrolizumab) recently approved by the FDA is used to treat patients with advanced hepatocellular carcinoma who have received sorafenib treatment, but the objective response of the patients is less than 20%

Method used

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  • Application of artemisia absinthium extract to preparing anti-hepatoma medicine
  • Application of artemisia absinthium extract to preparing anti-hepatoma medicine
  • Application of artemisia absinthium extract to preparing anti-hepatoma medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A prepares the operation steps of Artemisia chinensis ethanol extract as follows:

[0054] (1) Artemisia annua Central Asia was purchased from Jiamikang Pharmacy of Alikang Uygur Pharmaceutical Technology Co., Ltd., and the aerial part of Artemisia annua Central Asia was taken and crushed. Extract overnight at 4°C, centrifuge at 8000rpm for 20min, and take the precipitate;

[0055] (2) Mix the precipitate obtained in step (1) with water according to the mass volume ratio of 1g: 10ml, reflux extraction in a water bath at 60°C for 2h, centrifuge at 8000rpm for 20min, and take the precipitate;

[0056] (3) The precipitate that step (2) obtains is extracted 3 times with 85% ethanol backflow; ℃ water bath reflux extraction for 2h;

[0057] (4) Suction filter the solution after the third reflux extraction in step (3), take the supernatant, filter, and concentrate by rotary evaporation until it has no ethanol smell, to obtain the extract; then freeze-dry the extract in vacuu...

Embodiment 2

[0070] Preparation of anti-liver cancer drugs:

[0071] Weigh 100 mg of the alcoholic extract of Artemisia chinensis prepared above, add it to 1000 ml of water, heat to dissolve, put it into a bottle, seal it, and sterilize it to prepare a 0.1 mg / ml anti-liver cancer drug.

[0072] Weigh 200mg of the above-prepared Artemisia oleifera petroleum ether extract, add it to 1000ml of water, heat to dissolve, put it into a bottle, seal it, and sterilize it to prepare a 0.2mg / ml anti-liver cancer drug.

[0073] Weigh 300 mg of the above-prepared ethyl acetate extract of Artemisia chinensis, add it to 1000 ml of water, heat to dissolve, put it into a bottle, seal it, and sterilize it to prepare a 0.3 mg / ml anti-liver cancer drug.

Embodiment 3

[0075] Screening of the prepared ethanol extract of Artemisia annua, petroleum ether, and ethyl acetate for inhibiting the growth of liver cancer cells in vitro:

[0076] Screening method: BEL-7404 and H22 cells were treated with different doses (25, 75 and 150 μg / mL) of AAEE, AAEW-Pe and AAEE-Ea, and the changes in cell morphology were observed after 24, 48 and 72 hours (such as figure 1 shown in A). Compared with untreated cells, after AAEE, AAEE-Pe, AAEW-Ea treatment, the cells became round, shrunk and the number of cells decreased.

[0077] The cell activity of AAEW, AAEW-Pe, AAEW-Ea after treatment of BEL7404 and H22 cells for 24h, 48h and 72h was detected by MTT method, and it was found that AAEE, AAEE-Pe, AAEE-Ea decreased BEL7404 in a dose-dependent and time-dependent manner. and the activity of H22 cells (such as figure 1 shown in B). Calculate the half maximal inhibitory concentration (IC50) of AAEE, AAEE-Pe, AAEE-Ea to BEL7404 and H22 cells at 24h, 48h and 72h, a...

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Abstract

The invention discloses application of artemisia absinthium extract to preparing anti-hepatoma medicine. According to the application of the artemisia absinthium extract to preparing the anti-hepatomamedicine, hepatoma cell apoptosis is caused by inducing endoplasmic reticulum stress and mitochondria dependency path through active oxygen generation in artemisia absinthium alcohol extract and petroleum-ether and ethyl-acetate extract, and growth of hepatoma cells is inhibited; the inhibition rate of the artemisia absinthium alcohol extract for internal tumor growth reaches 60% or above, and the inhibition rate of the petroleum-ether and ethyl-acetate extract for tumor growth reaches 80% or above.

Description

technical field [0001] The invention belongs to the application of Artemisia annua, in particular to the application of Artemisia annua extract in the preparation of anti-liver cancer drugs. Background technique [0002] According to the 2018 global cancer statistics, liver cancer is the sixth most common cancer in the world and the fourth leading cause of cancer death in the world. The mortality rate of liver cancer accounts for 8.2% of the total number of cancer deaths. In China, liver cancer is the third leading cause of cancer-related death. According to statistics, the new cases of liver cancer in China account for 50% of the new cases of liver cancer in the world. More than 90% of liver cancer patients are hepatocellular carcinoma (hepatocellular carcinoma, HCC), currently liver resection is the main treatment for hepatocellular carcinoma, but less than 30% of patients meet the treatment criteria for liver resection, which is due to the diagnosis of liver cancer patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P35/00
CPCA61K36/28A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P35/00
Inventor 李金耀马荣斌魏仙仙李金玉
Owner XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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