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Sperm-motility grading counting method

A counting method and motility technology, applied in the field of sperm counting, to avoid repeated counting and omission, avoid counting interference, improve operation difficulty and data accuracy

Inactive Publication Date: 2019-04-19
潘世鑫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fifth edition reduces the difficulty of counting motility, but how to count motile sperm more accurately and without repeated counting remains a difficult problem in the operation of artificial sperm counting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Semen Motility Grading and Counting Operation

[0036] 1. Experimental instrument: BX41 Olympus microscope

[0037] Modified Neubauer hemocytometer and matching coverslip

[0038] glass slide

[0039] 22mm×22mm cover glass

[0040] 2. Experimental reagents: Fixed diluent (solution A):

[0041] Dissolve 50g NaHCO in 1000ml pure water 3 and 10ml of 35% (v / v) formaldehyde solution

[0042] TBS buffer solution (solution B) The TBS buffer solution is Tris-HCl buffered saline solution: the formula is 8 grams of NaCL, 0.2 grams of KCL, 3 grams of Trise base, add 800 mL of pure water, adjust the pH to 7.4 with concentrated hydrochloric acid, and then Add pure water to 1000mL.

[0043] 3. Counting method:

[0044] as attached figure 1 As shown, for the counting improved Neubauer hemocytometer, the central square is divided into 9 large squares from 1 to 9, and each large square is used as shown in the attached figure 1 labels to distinguish. The No. 5 squ...

Embodiment 2

[0078] Embodiment 2: use the step of embodiment 1 and the grading result comparison of WTO recommended method

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Abstract

The invention discloses a sperm-motility grading counting method, and particularly relates to the technical field of sperm counting. The sperm-motility grading counting method mainly includes the steps that seminal fluid is diluted with fixing diluent and TBS buffer liquid respectively, counting is carried out respectively, and therefore the A-liquid total sperm number and the B-liquid total NP and IM number are obtained; after errors are verified, the number of active (PR) sperms can be obtained through calculation, and the motility of the sperms is determined. By means of the sperm-motilitygrading counting method, sperm movement and the interference in counting when the sperm movement grade is judged can be avoided, the grading difficulty of the motility is reduced to a great degree, and accurate motility grading results are obtained.

Description

technical field [0001] The invention relates to the field of sperm counting, in particular to a method for graded counting of sperm motility. Background technique [0002] Manual counting in semen analysis is not as simple as instrumental counting, but manual counting is still the gold standard for semen analysis. Although manual counting is accurate, the operation is cumbersome. Especially in the counting of sperm motility, the examiner is required to count quickly, and the sperm cannot be counted repeatedly. However, the sperm moves quickly and the direction is uncertain, which will inevitably cause errors. The results will vary greatly. According to the requirements of the WHO "Human Semen Inspection and Processing Laboratory Manual Fourth Edition", the activity is graded by abcd, and it is difficult to distinguish grade a from grade b. Today, the WHO has published the fifth edition of the "Laboratory Manual for the Examination and Processing of Human Semen", which recl...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCG01N15/10G01N2015/1024G01N15/01
Inventor 潘世鑫王萍李福贵黄金山
Owner 潘世鑫
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