A method for reducing calcium and magnesium ion content in seawater, microbial scale inhibitor and use
A technology of calcium and magnesium ions and microorganisms, applied in seawater treatment, water pollutants, general water supply conservation, etc., can solve problems such as expensive, chemical methods, adverse effects on natural and human environment, and complexity, achieve dense structure, prevent reverse osmosis membranes, etc. The effect of scaling, reducing production and maintenance costs
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Embodiment 1
[0028] Proceed as follows:
[0029] 1. Expanded culture of water-producing Alkaline bacteria
[0030] 1.1 Take out the strains of Alcalibacterium producing water that are stored in 2216E liquid medium containing 20% glycerol at a storage temperature of -80°C, place them at room temperature, recover their vitality, and then inoculate 100 μl of the strains into 2216E liquid In the culture medium, at 37°C, shake culture at 120r / min for 12 hours to obtain the bacterial liquid; use sterile seawater to adjust the bacterial density in the bacterial liquid to 10 8 CFU / ml to obtain microbial antiscalant.
[0031] 1.2 Add the density-adjusted bacterial solution (ie microbial antiscalant) to seawater. Seawater was collected from the East China Sea, filtered by sedimentation, and sterilized in a sterilizing pot at 121°C for 15 minutes to make sterile seawater. The sterile seawater was put into a sterile Erlenmeyer flask; 1ml of bacteria solution (i.e. microbial scale inhibitor), the ...
Embodiment 2
[0043] After the microbial scale inhibitor was added to the seawater in Example 1, the concentration of viable bacteria in the seawater was detected immediately. Doubling dilution was performed with sterile seawater, and 100 μl of the diluted seawater was spread on 2216E solid medium, cultured at 25°C for 72 hours, and the number of colonies was counted to obtain a bacterial concentration of 100,000 CFU / ml in the seawater of Example 1.
Embodiment 3
[0045]Take out the strains of Alcalibacterium in water that are stored in 2216E liquid medium containing 20% glycerol at a storage temperature of -80°C, place them at room temperature, recover their vitality, and then inoculate 100 μl of the strains into 2216E liquid culture medium, at 30°C, 120r / min shaking culture for 16 hours, the bacterial density was greater than 10 8 CFU / ml microbial antiscalant.
[0046] In summary, Alcaligenes aquatilis can use calcium and magnesium ions in seawater to form stable organic-inorganic composite biodeposits with a calcite structure, which greatly reduces calcium and magnesium ions in seawater and avoids the occurrence of reverse osmosis seawater. The fouling problem of reverse osmosis membrane occurs during the desalination process. This method of reducing the content of calcium and magnesium ions in seawater is simple to operate, less in dosage, non-toxic and non-polluting, and has no special requirements for reverse osmosis seawater d...
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