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Method for extracting and separating active ingredients of hpyerglycemic effective part of longan leaves

A technology of active ingredients and effective parts, applied in the field of separation of plant active ingredients, can solve the problem of not many longan leaves, and achieve the effects of abundant sources, reasonable process design and low price

Pending Publication Date: 2019-04-19
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many research reports on longan leaves in China, mainly focusing on the extraction of flavonoids, and there is no extraction and separation method for its effective parts or active ingredients for lowering blood sugar

Method used

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  • Method for extracting and separating active ingredients of hpyerglycemic effective part of longan leaves
  • Method for extracting and separating active ingredients of hpyerglycemic effective part of longan leaves
  • Method for extracting and separating active ingredients of hpyerglycemic effective part of longan leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The preparation method of the active ingredient of the effective part of longan leaves for lowering blood sugar comprises the following steps:

[0031] S1. Get 10kg of dried longan leaves, pulverize and extract with 95% ethanol and 50% ethanol successively by percolation, and recover the ethanol under reduced pressure from the percolation extract to obtain extract;

[0032] S2. adding water to the ethanol extract to suspend, sequentially extracting 3 times with petroleum ether and ethyl acetate respectively to obtain the corresponding extract;

[0033] S3. Separating and concentrating the extracts under reduced pressure to recover the solvent to obtain the petroleum ether part and the ethyl acetate part respectively;

[0034] S4. Take the part of petroleum ether, go through silica gel column chromatography, use petroleum ether-ethyl acetate (100:0→0:100) gradient elution, detect and identify by TLC, develop with different developing agents, develop color with iodine vap...

Embodiment 2

[0042] The preparation method of the active ingredient of the effective part of longan leaves for lowering blood sugar comprises the following steps:

[0043] S1. Take 10 kg of dry longan leaves, pulverize them and extract them by percolation with 50% ethanol, combine the percolation extracts, reclaim ethanol under reduced pressure to obtain extract;

[0044] S2. adding water to the ethanol extract to suspend, sequentially extracting 3 times with petroleum ether and ethyl acetate respectively to obtain the corresponding extract;

[0045] S3. Separating and concentrating the extracts under reduced pressure to recover the solvent to obtain the petroleum ether part and the ethyl acetate part respectively;

[0046] S4. Take the part of petroleum ether, go through silica gel column chromatography, use petroleum ether-ethyl acetate (100:0→0:100) gradient elution, detect and identify by TLC, develop with different developing agents, develop color with iodine vapor, and the same spot ...

Embodiment 3

[0054] The preparation method of the active ingredient of the effective part of longan leaves for lowering blood sugar comprises the following steps:

[0055] S1. Get 10kg of dry longan leaves, pulverize and extract with absolute ethanol percolation, and the percolation extract decompresses and reclaims ethanol to obtain extract;

[0056] S2. adding water to the ethanol extract to suspend, sequentially extracting 3 times with petroleum ether and ethyl acetate respectively to obtain the corresponding extract;

[0057] S3. Separating and concentrating the extracts under reduced pressure to recover the solvent to obtain the petroleum ether part and the ethyl acetate part respectively;

[0058] S4. Take the part of petroleum ether, go through silica gel column chromatography, use petroleum ether-ethyl acetate (100:0→0:100) gradient elution, detect and identify by TLC, develop with different developing agents, develop color with iodine vapor, and the same spot Fractions with the s...

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Abstract

The invention discloses active ingredients of hpyerglycemic effective part which has good hypoglycemic effect by extracting and separating from the longan leaves by using modern separation means. Theactive ingredients include lupinol, ethyl gallate, kaempferol, luteolin, and quercetin. The active ingredients can be obtained by pulverizing the longan leaves and then extracting with ethanol, a percolate solution is decompressed to recover ethanol, and then an extract is obtained, and the extract is suspended by adding water, the petroleum ether and ethyl acetate are used for extraction to obtain a corresponding petroleum ether part and an ethyl acetate part, and the petroleum ether part and ethyl acetate part are taken and separated and purified by a silica gel column chromatography in a repeated mode and recrystallized. The pharmacological experiments show that the obtained compound has the function of controlling alpha-glucosidase activity.

Description

technical field [0001] The invention relates to the technical field of separation of effective plant components, in particular to a method for extracting and separating the active components of the effective part of longan leaves for lowering blood sugar. Background technique [0002] Longan leaves are leaves or buds of Longan (Dimocarpus longan Lour.). Sweet, light, neutral in nature, it has the effects of clearing away heat, promoting dampness and detoxification. It is mainly used to treat colds, fever, malaria, boils, eczema, etc. Longan originates in the subtropical regions of southern my country and northern Vietnam, and has been cultivated in my country for more than 2,000 years. my country's longan cultivation area and output both rank first in the world. Every year after the longan is picked, fruit farmers cut off the branches of the trees, and a large number of leaves are discarded or burned, resulting in waste of resources and environmental pollution. Longan lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P3/10A61K127/00
CPCA61K36/77A61K2236/33A61K2236/53A61K2236/55
Inventor 梁洁黄春燕麦嘉妮柳贤福
Owner GUANGXI UNIV OF CHINESE MEDICINE
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