Building method for UPLC spectrums of semen vaccariae and fried semen vaccariae, and application

A technique of frying Wang Bu Liu Xing and Wang Bu Liu Xing, which is applied to the establishment and application of UPLC atlas of Wang Bu Liu Xing and fried Wang Bu Liu Xing, can solve the problems of increasing the dissolution rate of water decocting, and shorten the detection time. , Good repeatability and accuracy, UPLC detection good separation effect

Active Publication Date: 2019-04-16
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still no conclusion about how the main medicinal ingredients change during the processing and dissolution process and whether the dissolution rate of water decoction can increase.
And at present, there are few research literatures on the characteristic map of (fried) Wang Buliuxing

Method used

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  • Building method for UPLC spectrums of semen vaccariae and fried semen vaccariae, and application
  • Building method for UPLC spectrums of semen vaccariae and fried semen vaccariae, and application
  • Building method for UPLC spectrums of semen vaccariae and fried semen vaccariae, and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1 material

[0027] 1.1 Instruments and reagents

[0028] Instruments: Waters high-performance liquid chromatography (Waters company), YMC TriartC18 (2.1 × 100mm, 1.9 μm), one-millionth balance (Mettler-Toledo, ME204E), one-millionth balance (Metro Teller-Toledo, XP26).

[0029] Reagents: ethanol (Tianjin Yongda Chemical Reagent Co., Ltd.) is analytical grade, methanol (Guangdong Guanghua Technology Co., Ltd.) is analytical grade, acetonitrile (Merck Co., Ltd.) is chromatographic grade, water is ultrapure water (Merck Incorporated, Mili-QDirect).

[0030] Test drug: Erythrinaline (Shanghai Yuanye Biotechnology Co., Ltd., content: 98.0%, batch number: P26N6F6550), blaliculin glycosides (China National Institutes for Food and Drug Control, content: 99.7%, batch number: 111853-201704) .

[0031] 2. Methods and Results

[0032] 2.1 Wangbuliuxing UPLC characteristic map

[0033] 2.1.1 Chromatographic conditions

[0034] Use YMCC18 (100mm×2.1mm, 1.9μm) as the chromatog...

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Abstract

The invention provides a building method for UPLC spectrums of semen vaccariae and fried semen vaccariae, and an application. The building method for the UPLC spectrums of the semen vaccariae and thefried semen vaccariae comprises the following steps: a test solution is prepared: test powder of the semen vaccariae and the fried semen vaccariae is taken, 75% ethanol is added, ultrasonic treatmentis performed, filtering is performed, and a filtrate is taken, so that the test solution is obtained; and high performance liquid chromatograph detection is performed: a Waters high performance liquidchromatograph is adopted for detecting the test solution, and the UPLC spectrums are built. During identification, vaccarin of the UPLC spectrums is used as a reference peak, and the relative peak area of a characteristic peak with relative retention time in a range of 1.25+ / -10% is calculated, wherein the relative peak area of the semen vaccariae is in a range of 0.068-0.149, and the relative peak area of the fried semen vaccariae is in a range of 0.183-0.411.

Description

technical field [0001] The present invention relates to a method for establishing UPLC spectra of Wang Buliuxin and Chao Wang Buliuxin and an application method for distinguishing Wang Buliuxin and Chao Wang Buliuxin. Background technique [0002] Wangbuliu is the dry mature seed of Vaccaria segetalis (Neck.) Garcke, a caryophyllaceae plant. . Buliuxing contains a variety of chemical components, such as triterpenoid saponins, cyclic peptides, flavonoids, alkaloids, phenolic acids, steroids and volatile oils, among which triterpenoid saponins are the most abundant, followed by flavonoids and cyclic peptides. [0003] At present, the cooking method of Wang Buliuxing in the Pharmacopoeia is stir-frying. Since ancient times, there has been a saying that "every child must be fried", but it is currently believed that the bioavailability of sub-class traditional Chinese medicine may increase after frying. There are not many reports on the research on the processing mechanism of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 黎晓丽吴文平杨文惠梁慧杨晓东罗文汇罗宇琴陈向东
Owner GUANGDONG YIFANG PHARMA
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