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Set of HDACs nucleic acid aptamers

A nucleic acid aptamer and aptamer technology, applied in the fields of genetic engineering, immunology and oncology, to achieve the effect of small molecular weight, easy synthesis and modification, and easy synthesis and preservation

Inactive Publication Date: 2019-04-12
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening of HDACs aptamers. If aptamers that can specifically bind HDACs can be screened, it will undoubtedly provide a broader platform for the detection of HDACs.

Method used

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  • Set of HDACs nucleic acid aptamers
  • Set of HDACs nucleic acid aptamers
  • Set of HDACs nucleic acid aptamers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] The HDACs nucleic acid aptamer of the present invention takes HDAC1 as an example, and the specific technical scheme is:

[0042] 1. Optimize the annealing temperature in the PCR program

[0043] The annealing temperature in the PCR program of the ssDNA library has a greater impact on the entire screening process. Therefore, in order to achieve the highest efficiency of PCR amplification in each round of screening, the annealing temperature in the PCR program of the ssDNA library needs to be optimized before the start of the screening. The specific PCR system is shown in Table 1:

[0044] Table 1 The optimized reaction system of PCR annealing temperature

[0045] Reaction component

volume

10x PCR Buffer

2 μL

dNTP (2.5 mM)

1.6 μL

FP (10 μM)

0.8 μL

RP (10 μM)

0.8 μL

Taq DNA Polymerase (5 u / μL)

0.2 μL

Template

2 μL

H 2 O

12.6 μL

[0046] The specific steps are as follows:

[0047] Prepare 12 tubes of PCR reaction mixture according to the table, mix well, a...

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Abstract

The invention discloses a set of HDACs nucleic acid aptamers, and belongs to the technical field of genetic engineering, immunology and oncology. The aptamers are any one or several nucleotide sequences as shown in SEQ ID NO: 1-5. The nucleic acid aptamers of the invention are obtained by screening with a protein SELEX technology, have a small molecular weight, are easy to synthesize and modify, and can recognize HDACs with high specificity and bind with the HDACs with high affinity. The aptamers do not bind with other protein, have no immunogenicity, are stable and easy to modify, and are easy to synthesize and preserve. An HDACs detection kit and reagent papers prepared from the aptamers can detect the HDACs with high sensitivity, and are easy to optimize. The aptamers are used in tumordiagnosis, evaluation of tumor prognosis and monitoring, and tumor diagnosis.

Description

Technical field [0001] The invention belongs to the technical fields of genetic engineering, immunology and oncology, and particularly relates to a set of HDACs nucleic acid aptamers. Background technique [0002] Cancer is generally considered to be a genetic defect disease caused by the activation of oncogenes or the loss of tumor suppressor genes. However, epigenetic changes, including histone acetylation, also contribute to tumor development. Histone acetyltransferases and deacetylases (HDACs) play an important role in chromatin structure modification and gene expression. The inactivation of histone acetyltransferase is related to tumorigenesis. Abnormal HDAC activity is considered to be the key to tumor formation, because the excessive and inappropriate activity of HDAC inhibits the transcription of tumor suppressor genes. [0003] Nucleic acid aptamers are short single-stranded DNA (ssDNA) or RNA molecules that are screened and used to bind to specific targets. They are ea...

Claims

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Application Information

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IPC IPC(8): C12N15/115G01N33/574G01N33/573
CPCC12N15/115C12N2310/16G01N33/573G01N33/57484G01N2333/916
Inventor 赵永祥张志勇张坤周素芳黄勇阳诺
Owner GUANGXI MEDICAL UNIVERSITY
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