High-throughput three-dimensional cell pellet culture and in-situ drug sensitivity test method

Abstract

The invention discloses a method for high-throughput three-dimensional cell pellet culture and in-situ drug sensitivity test, and belongs to the field of biotechnology. The method is as below: first,forming an array of droplets on a super-hydrophobic micro-pit array chip; second, forming a three-dimensional cell pellet array on a super-hydrophobic micro-pit array chip; third, adding algin into the super-hydrophobic micro-pit array chip, and fixing the cell pellets in the micro-pit; and fourth, conducting in-situ drug sensitivity test on the super-hydrophobic micro-pit array chip. The method can realize the simultaneous loading of each micro-pit of the chip by using a self-built humidification and aligning device, simply and efficiently realizes the construction of the high-throughput three-dimensional cell pellet micro-array by using hanging drop culture and fluid infusion operation, establishes a method for detecting cell activity on the chip, and utilizes a self-established on-chipcell activity detection method to realize the on-chip drug susceptibility test, thereby avoiding the transfer operation of the cell pellets and the resulting loss and damage of the cell pellets.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to cell culture, in particular to a three-dimensional cell bead culture and in-situ drug sensitivity testing method based on a superhydrophobic micropit array chip, which is easy to operate and high in throughput. Background technique [0002] In recent years, three-dimensional cell spheroids are generally considered to be a cell culture model that is closer to the tissue structure in vivo, and it is expected to become a golden model for in vitro drug screening, filling the huge gap between two-dimensional conventional cell culture and animal experiments. At present, one type of method is to obtain cell pellets through rotary culture and non-adherent culture. For example, the ultra-low adsorption culture dish of Corning Company of the United States can prevent cells from adhering to the wall and promote cells to form three-dimensional pellets. However, the uniformity of the cell pellets fo...

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Problems solved by technology

[0004] In order to solve the problems of high cost, cumbersome operation, and difficulty in realizing in situ drug sensitivity in the current methods of constructing high-throughput three-dimensional cell bead arrays, the present invention constructs a low-cost, easy-to-operate high-throughput three-dimensional cell Ball Array Model

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