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Method for supercritical extraction of astaxanthin

A supercritical extraction and astaxanthin technology, which is applied in the production of bulk chemicals, organic chemistry, etc., can solve the problems of long extraction time and low extraction rate, and achieve the effects of high extraction efficiency, simple operation and short preparation time.

Inactive Publication Date: 2019-04-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low extraction rate and long extraction time in the extraction of astaxanthin from Haematococcus pluvialis, the present invention provides a method for supercritical extraction of astaxanthin, which is stable and easy to operate, and can improve astaxanthin The extraction rate and product quality are economical and environmentally friendly, and the extract can be directly prepared astaxanthin soft capsules, which are safe and non-toxic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Experimental materials: Haematococcus pluvialis, purchased from Shandong Jinjing Biotechnology Co., Ltd., the particle size of Haematococcus pluvialis granulated with ultrafine powder broken wall is 20-30 mesh; CO 2 , with a purity of 99.9%, purchased from Guangzhou Gas Factory; standard astaxanthin, purchased from Chengdu Purifa Biotechnology Co., Ltd., acetone, 95% ethanol, methanol, etc., all of analytical purity, purchased from Tianjin Damao Chemical Reagents factory.

[0019] Experimental method: Supercritical CO 2 In the extraction kettle of the extraction equipment, the extraction kettle, separation kettle I, and separation kettle II are heated respectively, and the refrigerator is refrigerated. Boost the pressure of the extraction kettle, the separation kettle I, and the separation kettle II. When the pressures of the extraction kettle, the separation kettle I, and the separation kettle II rise to the set values ​​respectively, the circular extraction starts, a...

Embodiment 2

[0022] On the basis of Example 1, accurately weigh 100 g of Haematococcus pluvialis that has been broken and granulated, and put it into the extraction kettle. The temperature of the extraction kettle is 45 ° C, the extraction time is 90 minutes, and the CO 2 The flow rate is 30Kg / h, the pressure of the separation tank I is 11MPa, the temperature of the separation tank I is 45°C, the pressure of the separation tank II is 4MPa, and the temperature of the separation tank II is 40°C. The influence of the pressure of the extraction tank on the extraction rate of astaxanthin is shown in Table 1:

[0023] Table 1

[0024] Extraction kettle pressure (MPa)

[0025] It can be seen from Table 1 that the extraction rate of astaxanthin increases continuously with the increase of the pressure of the extraction kettle. When the pressure of the extraction kettle is 65MPa, the extraction rate of astaxanthin is greatly improved. It can be seen that the extraction pressure has a sign...

Embodiment 3

[0027] On the basis of Example 1, accurately weigh 100 g of Haematococcus pluvialis that has been broken and granulated, and put into the extraction kettle, the pressure of the extraction kettle is 65Mpa, the extraction time is 90min, and the CO 2 The flow rate is 30Kg / h, the pressure of the separation tank I is 11MPa, the temperature of the separation tank I is 45°C, the pressure of the separation tank II is 4MPa, and the temperature of the separation tank II is 40°C. The influence of the temperature of the extraction tank on the extraction rate of astaxanthin is shown in Table 2:

[0028] Table 2

[0029] Extraction kettle temperature (°C)

[0030] The temperature of the extraction kettle is also supercritical CO 2 An important factor affecting extraction, its influence is manifested as a double action. On the one hand, the increase in temperature increases the volatility and diffusion coefficient of the extracted solute, thereby increasing the concentration of th...

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Abstract

The invention discloses a method for supercritical extraction of astaxanthin, which comprises the following steps of: granulating haematococcus pluvialis with wall-broken superfine powder, loading into an extraction kettle of a supercritical CO<2> extraction device, respectively raising temperature and pressure of the extraction device to an extraction condition, starting circular extraction, keeping constant temperature and pressure until required extraction time is kept, performing extraction by taking supercritical CO<2> as an extraction agent and edible oil as an entrainer in an extractionprocess, and discharging and weighing from a separation kettle after the extraction is finished so as to calculate an astaxanthin extraction rate. By the adoption of the method for the supercriticalextraction of the astaxanthin, the edible oil is used as the entrainer, the astaxanthin in the haematococcus pluvialis is extracted by using a supercritical CO<2> extraction technology, and a supercritical CO<2> extraction sample can be directly prepared into an astaxanthin soft capsule according to a conventional soft capsule filling process. A preparation method provided by the invention has theadvantages of good stability, simple operation, short preparation time, high extraction efficiency, capability of preventing the astaxanthin from being oxidized and degraded and the like; and the astaxanthin soft capsule prepared by the extract is safe and nontoxic.

Description

technical field [0001] The invention relates to the technical field of extracting chemical components of Chinese medicinal materials, in particular to a method for supercritically extracting astaxanthin. Background technique [0002] Astaxanthin belongs to carotenoids, which has a strong ability to scavenge free radicals and quench reactive oxygen species, and it also has many physiological effects such as inhibiting tumorigenesis and enhancing immune function. The antioxidant capacity of astaxanthin is 10 times that of β-carotene and 500 times that of vitamin E. It is called "super vitamin E" and "super antioxidant". At present, astaxanthin has been widely used in food, medicine, cosmetics and breeding industries. Natural astaxanthin mainly exists in Haematococcus pluvialis, and its content can reach 2-6% of stem cells, so pluvialis is regarded as the "concentrated product" of natural astaxanthin. Haematococcus pluvialis was approved as a new food resource by the State Fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16Y02P20/54
Inventor 段明慧葛发欢李海池
Owner SUN YAT SEN UNIV
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