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A method for constructing an animal model for evaluating the immune injury of mice caused by cigarette smoke

A technology of cigarette smoke and animal models, applied in the field of biomedicine, can solve problems such as the composition and function of the immune system

Inactive Publication Date: 2021-07-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition and function of the immune system of the body are complex, and there is no effective animal model to comprehensively evaluate the impact of smoking on the immune system and function of the animal body.

Method used

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  • A method for constructing an animal model for evaluating the immune injury of mice caused by cigarette smoke
  • A method for constructing an animal model for evaluating the immune injury of mice caused by cigarette smoke
  • A method for constructing an animal model for evaluating the immune injury of mice caused by cigarette smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 The establishment of the biological effect model of mouse smoking concentration and time and the experimental model of immune injury

[0055] How to evaluate the biological effects of smoking is the first problem related to the impact of subsequent smoking on the immune system, that is, the function of the body. Because smoking first affects the lungs, and the body's immune system is distributed in every organ and tissue of the body including the lungs, therefore, this embodiment adopts the method of scoring the pathological disease activity index (DAI) of the mouse lungs after smoking. The relationship between the dose and time of smoking and the effect is used for the evaluation of the impact on the immune system and function.

[0056] The disease activity index (DAI) scoring method and standard of lung pathology in mice after smoking are as follows:

[0057] Alveolar integrity score A1 (0-3 points): 0 points for complete alveoli (-), 1 point for mild damag...

Embodiment 2

[0074] Example 2 Using Animal Models to Evaluate the Effects of Smoking on the Regional Immunity of the Mice's Lungs

[0075] The smog in this example is derived from the control cigarette (production batch number 20150225-0) provided by Jiangxi China Tobacco Industry Co., Ltd., and the smog is input into the animal smoking gas poisoning instrument (HRH-CSED) through the intelligent smoking machine (HRH-SM120). -K) in the sealed poisoning cabin, and set the required cigarette smoke concentration in the poisoning cabin space

[0076] Put the animals in the animal smoking gas poisoning instrument (HRH-CSED-K), set the smoke concentration of the mice to be poisoned at 60%, smoke 1 time / day, respectively smoke 10min / time / day, 20min / time / day , 40min / time / day; the normal control group did not smoke. Smoking was stopped after 1 month, and corresponding experiments were carried out. In this embodiment, the change of the cell content and the expression of cytokines in the mouse alveola...

Embodiment 3

[0085] Embodiment 3 Utilizes animal model to evaluate the impact of smoking on body weight and immune organs of mice

[0086] The present embodiment evaluates that under the condition of 60% smoke concentration in mice, the smoking time is divided into 10min / time / day, 20min / time / day, 40min / time / day, and the body weight and immune organs of mice after smoking for 1 month Impact. The normal control group did not smoke. Smoking was stopped after 1 month, and corresponding experiments were carried out to measure the changes in the body weight of the mice; the spleen and thymus were removed after the mice were sacrificed. Analytical balance weighing, calculation of immune organ coefficient.

[0087] After the mice smoked, the weights and coefficients of the immune organs thymus and spleen of the mice in each group are shown in Table 3-5. It can be seen from Table 3-5 that under the condition of smoke concentration of 60%, smoking 20min / time / day, 40min / time / day, and smoking for 3...

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Abstract

A method for constructing an animal model for evaluating the immune injury of mice by cigarette smoke, comprising the following steps: placing experimental mice in a smoking gas poisoning instrument, setting the concentration of cigarette smoke for mice to be poisoned to 60%, and 1 Smoking 10-40min / time / day, continuous smoking for 30 days, to obtain an animal model for evaluating the immune injury of mice caused by cigarette smoke. The establishment of this animal model helps to understand and systematically evaluate the impact and characteristics of smoking on the immune system of animals, and can also provide experimental data for the judgment of tobacco harm reduction, and then develop low-toxicity cigarettes to provide harm reduction for those who cannot quit smoking. product.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for constructing an animal model for evaluating the immune injury of mice caused by cigarette smoke. Background technique [0002] Smoking is a group phenomenon in all countries in the world. In recent years, research on the health effects of smoking has attracted more and more attention. The body's immune system has important physiological functions and is the defender of the body's health. Given this, it is desirable to understand the mechanisms underlying whether and how smoking affects the immune system. The earlier foreign studies on the effects of tobacco on the body's immunity began in the late 1970s. They mainly observed the changes in local immunity around the respiratory tract of smokers, such as complement proteins, serum reactive proteins, immune complexes and lysozyme, especially for lung cancer. Clinical detection of some immune-related substances b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 胡显律苏加坤郭猛郭磊张铭健刘芳蔡继宝王全兴
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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