Establishment and selection method of recurrent selection population for high-quality multi-resistance tobacco new line breeding

A technology of reincarnation selection and new strains, which is applied in the field of reincarnation selection group creation and selection in the breeding of high-quality multi-resistant tobacco new strains, can solve the problems of reincarnation selection occupying a large area of ​​land and heavy workload, and achieve a high degree of gene aggregation, Enhanced dosage effect and excellent properties

Active Publication Date: 2022-04-19
HUBEI TOBACCO SCI RES INST
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since tobacco is a crop with a large plant spacing, and the number of seeds per fruit is large, the breeding targets involve a variety of traits, recurrent selection occupies a large area of ​​land, and the workload is heavy, so there has been no application of recurrent selection to the breeding of new high-quality multi-resistant tobacco varieties. to report
Although there are sporadic literatures that have been discussed in theory, they are all conceptualized and generalized. As for how to create a reincarnation selection group on tobacco, and how to select and deal with the reincarnation selection group, it is still unclear and has not been seen so far. Someone proposed a more practical and operable recurrent selection breeding program in tobacco breeding

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  • Establishment and selection method of recurrent selection population for high-quality multi-resistance tobacco new line breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Creation and selection of flue-cured tobacco reincarnation selection groups

[0045]In order to take into account the long-term and short-term breeding goals, when selecting the parents of the original population for the construction of flue-cured tobacco reincarnation, high quality and wide adaptability should be given priority to, and the resistance to all common and major diseases of flue-cured tobacco should be considered as much as possible. According to the breeding goal, the selected parents should include quality character parents, adaptive parents, high-yield and easy-to-roast parents, and disease-resistant parents. In order to use varieties in production with wide adaptability, the high-yield and easy-to-roast parents should include broad-leaved high-yield and easy-to-roast parents and multi-leaf high-yield and easy-to-roast parents, and the disease-resistant parents should include root disease-resistant parents, foliar disease-resistant Parents a...

Embodiment 2

[0054] Example 2: The introduction of heterologous germplasm in the recurrent selection population of flue-cured tobacco

[0055] In the summer of 2014, it was found that the reincarnation selection population had deficiencies in quality traits and resistance to black shank and bacterial wilt. Therefore, in the winter of the same year, the high-quality parent K326 was used as the female parent at the Sanya Southern Breeding Base, which is also resistant to black shank and bacterial wilt. Blight germplasm DB101 was used as the male parent, and the hybrid combination K326×DB101 was prepared.

[0056]In 2015, the first round of selection group C1 seeds obtained in 2014 and the F1 generation seeds of the hybrid combination K326×DB101 were sown in the Lichuan Experimental Base to raise seedlings, and the seedlings were transplanted into the field respectively. When flowering, select 500 tobacco plants with ideal growth and morphological traits in the first-round selection populat...

Embodiment 3

[0059] Example 3: Identification of excellent individual plants of flue-cured tobacco recurrent selection population

[0060] In 2013, 500 tobacco plants selected through disease resistance identification and also resistant to wildfire, black shank, common mosaic virus, climatic spot, root-knot nematode and red spot disease will be crossed between individual plants At the same time, the F3 generation seeds of the hybrids were obtained by selfing with Liuyancha, and the seeds were harvested per plant.

[0061] In 2014, 500 F3 generation seeds were sown in individual plants in the greenhouse seedbed of the Lichuan Experimental Base, and each individual plant had 50 tobacco seedlings, which was called a strain. When the seedlings are about 8 weeks old, inoculate and identify the resistance to angular spot disease. First, select the lines with better overall resistance, and then select the tobacco seedlings resistant to angular spot disease in the selected lines. A total of 451...

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Abstract

The present invention relates to a recurrent selection population creation and selection method for the breeding of high-quality multi-resistant tobacco new strains. First, the high-quality parent and the wide-fit parent are used as the female parent, and other parents are used as the male parent for hybridization, and then the high-quality parent combination and the high-quality parent are sequentially carried out. Widely suitable parental combination hybridization, double-cross combination containing high-yield and easy-to-modulate parents, double-cross combination without high-yield and easy-to-modulate parents, and cross-selected plants in the progeny population of compound hybrid combination to construct the basic population of recurrent selection. Then, self-crossing of selected strains within the population, identification of disease resistance and selection of superior strains, hybridization of superior strains of identification and screening of economic and quality traits, and cross-selection of selected strains were carried out to form the selection population for the next round. Each recurrent selection population can introduce heterologous germplasm, and can also extract the required individuals for strain identification. By adopting the method, a plurality of target traits can be simultaneously improved, and the breeding efficiency is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco breeding, and in particular relates to a recurrent selection group creation and selection method for breeding high-quality multi-resistant tobacco new strains. Background technique [0002] Tobacco is a self-pollinating crop. For a long time, the improvement of tobacco varieties has mainly relied on conventional cross-breeding methods, which are quite effective in achieving near-term breeding goals. However, due to the long-term crossbreeding of a small number of parents and the pedigree selection of offspring, it is difficult to combine multiple major genes that control the same target shape, and it is even more difficult to exert the potential effects of numerous micro-effect gene recombination and accumulation , resulting in the loss of a large number of favorable genes, and the genetic basis of the bred varieties is becoming narrower. For example, the main varieties of flue-cured tobacco in my...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 曹景林程君奇李亚培吴成林张俊杰
Owner HUBEI TOBACCO SCI RES INST
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