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A kind of rapid preparation method of Pleurotus eryngii liquid strain

A technology of liquid strains and Pleurotus eryngii, which is applied in the fields of botany equipment and methods, fungi, mushroom cultivation, etc., can solve the problems of high nutrients in the medium formula, large demand for strain tanks, poor transparency and inconvenience, and achieve Less residual nutrients, reasonable formula nutrients, and the effect of production capacity expansion and production demand

Active Publication Date: 2021-02-26
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One is that the existing method has a large demand for strain tanks and a long cultivation period;
[0005] The second is that the existing medium formula is too high in nutrients and too poor in transparency, which is not convenient for observation and judgment; after inoculation of cultivation bags, it is easy to be contaminated by bacteria, resulting in defects such as "pustules" bags

Method used

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  • A kind of rapid preparation method of Pleurotus eryngii liquid strain
  • A kind of rapid preparation method of Pleurotus eryngii liquid strain
  • A kind of rapid preparation method of Pleurotus eryngii liquid strain

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Embodiment 1

[0042] Such as figure 2 As shown, the embodiment of the present invention provides a kind of rapid preparation method of Pleurotus eryngii liquid spawn, comprising:

[0043] Prepare the first strain tank and the second strain tank after sterilization and cooling of the Pleurotus eryngii liquid strain that has completed aerobic culture for 8 days;

[0044] Connect the discharge port of the first strain tank to the sampling port of the second strain tank through the conversion joint to inoculate the Pleurotus eryngii liquid strain, and connect 100L each time to the second strain tank.

[0045] The second seed tank was incubated at 22°C for 3 days.

[0046] Wherein, the structure of the conversion joint is:

[0047] Feed pipe 101, the liquid strain cultivated in the first strain tank is sent in the discharge pipe 102;

[0048] A plurality of discharge pipes 102 are arranged at intervals, and the discharge pipe 102 and the feed pipe 101 are parallel to each other, so that the ...

Embodiment 2

[0063] The present embodiment provides a kind of rapid preparation method of Pleurotus eryngii liquid spawn, comprising:

[0064] Step 1, prepare and configure the culture medium according to the formula of the Pleurotus eryngii liquid strain culture medium. The formula of Pleurotus eryngii liquid strain culture medium used in the present embodiment includes white sugar 0.85-1.1%, yeast extract powder 0.08-0.12%, soybean meal powder 0.1-0.2%, potassium dihydrogen phosphate 0.05-0.08% by tank volume % and magnesium sulfate 0.05-0.08%. Preferably, 0.07-0.13% of zinc sulfate, 0.08-0.12% of calcium sulfate and 0.008-0.012% of defoamer can be added based on the above formula based on tank volume.

[0065] Step 2, the preparation method of Pleurotus eryngii liquid strain is:

[0066] Dissolving white sugar, magnesium sulfate and potassium dihydrogen phosphate in water; adding soybean meal powder and yeast extract powder in water and stirring to dissolve, removing foam; mixing the ...

Embodiment 3

[0078] The rapid preparation method of Pleurotus eryngii liquid strain comprises:

[0079] Step 1, prepare and configure the culture medium according to the formula of the Pleurotus eryngii liquid strain culture medium. The formula of Pleurotus eryngii liquid culture medium used in the present embodiment is calculated by tank volume.

[0080] Step 2, the preparation method of Pleurotus eryngii liquid strain is:

[0081]Dissolve white sugar, magnesium sulfate, potassium dihydrogen phosphate, zinc sulfate (optional), and calcium sulfate (optional) in water; add soybean meal powder and yeast extract powder in water and stir to dissolve, and remove foam; The above two solutions were mixed uniformly to prepare a medium liquid. The pH value of the medium liquid was adjusted to 7 with hydrochloric acid, and sterilized. Cool the sterilized medium liquid to 23°C in the first strain tank, insert Pleurotus eryngii strains, adjust the culture temperature to 23°C, and pass purified air ...

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Abstract

The invention discloses a method for quickly preparing liquid strains of Pleurotus eryngii, which comprises: preparing a first strain tank containing liquid strains of Pleurotus eryngii that has completed aerobic culture for 7-8 days and a second strain tank after sterilization and cooling. Two spawn tanks; connect the discharge port of the first spawn tank to the sampling port of the second spawn tank to inoculate the Pleurotus eryngii liquid spawn; cultivate the second spawn tank at 20-25°C for 2-3 days. The rapid preparation method of the Pleurotus eryngii liquid strain of the present invention greatly improves the utilization rate of the strain tank, reduces the number of tanks, reduces the space occupied by the tank, and improves the production efficiency under the same environmental conditions.

Description

technical field [0001] The invention relates to the technical field of edible fungus production. More specifically, the present invention relates to a method for rapidly preparing liquid spawn of Pleurotus eryngii. Background technique [0002] Pleurotus eryngii, the flesh is thick, crisp and tender, especially its stipe is dense, firm, milky white, all edible, known as "Pleurotus eryngii king" and "Scallop mushroom", with almond fragrance and abalone taste, It is suitable for various methods such as fresh food and processing, and is deeply loved by consumers. [0003] At present, the preparation process of Pleurotus eryngii liquid strains includes the steps of medium configuration, adjustment, sterilization, cooling, inoculation and cultivation, and the cultivation methods include bag cultivation, bottle cultivation and soil-covering cultivation. Among them, the technology of Pleurotus eryngii liquid strains has become more and more mature after years of development, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 刘启燕周洪英孙波吴洪丽黄云
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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