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Hyperproductive trichoderma reesei strain featuring improved beta-glucosidase activity

A technology of Trichoderma reesei and strains, applied in the direction of glycosylase, enzyme, hydrolase, etc., can solve the problem that Trichoderma reesei cannot reproduce sexually

Inactive Publication Date: 2019-04-05
INST FR DU PETROLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] To date, sexual reproduction of T. reesei has never been used as an enhancement tool because T. reesei has been thought to be incapable of sexual reproduction

Method used

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  • Hyperproductive trichoderma reesei strain featuring improved beta-glucosidase activity
  • Hyperproductive trichoderma reesei strain featuring improved beta-glucosidase activity
  • Hyperproductive trichoderma reesei strain featuring improved beta-glucosidase activity

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Experimental program
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Effect test

Embodiment 1

[0052] Embodiment 1: preparation according to the bacterial strain of the present invention (being the bacterial strain deposited with preservation number CNCM 1-5221) and determining its characteristics

[0053] 1. Preparation

[0054] The cellulase high producing strain RutC30 (or Rut-C30) (Montenecourt and Eveleigh, 1977) was crossed with strain A2 according to the protocol described by Seidl et al. Strain RutC30 (deposited with ATCC under accession number 56765) was obtained by three mutagenesis steps of strain QM6a and is one of the best producers of cellulases in the public domain (Peterson and Nevalainen, 2012).

[0055] After hybridization, multiple ascospore isolation campaigns were performed. 295 purified offspring were obtained. The latter were then screened in three steps. These successive screens made it possible to reduce the number of candidates from a few hundred to the individual with the highest cellulase production performance.

[0056] 2. Cellulase Prod...

Embodiment 2

[0079] Example 2: Preparation of a fertile cross between a bacterial strain according to the present invention (i.e. a strain deposited under the deposit number CNCM 1-5221) and a female sterile MAT1-2 bacterial strain

[0080] Strain RuA-149 was crossed with MAT1-2 female sterile Trichoderma reesei MAT1-2 from isolate QM6a. MAT1-1IDC1 strains A2 and QM6a (Linke et al., 2015) were used as positive controls (fertile cross). Hybridization by confrontation: The strains are inoculated in rows facing each other with approximately 1.5 cm between the two strains. Each row contained 4-6 inoculum of the same strain from conidia plates or cryovials. Hybrids are fertile when the ascospores are expelled from the ascothecia contained in the stroma. The expulsion of ascospores was observed by the presence of a yellowish substance in the orifices of the ascospores. Crosses were performed with strains RutC30, Cl847, QM9414 and Tu-6. The results are given in Table 2 below. The pedigrees o...

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Abstract

The present invention relates to a Trichoderma reesei strain which is hyper-producing and which has enhanced beta-glucosidase activity, as well as the use of said strain.

Description

technical field [0001] The present invention relates to a Trichoderma reesei strain with high yield and enhanced β-glucosidase activity, and the use of the strain. Background technique [0002] Trichoderma reesei (T. reesei) is a cellulolytic filamentous fungal species of Trichoderma that was discovered in the South Pacific during World War II. The fungus can secrete a large amount of cellulosic enzymes (cellulase and hemicellulase), which are currently mainly used in the production process of second-generation biofuels. Indeed, the enzymes produced by this fungus are particularly suitable for converting plant biomass material into industrially useful bioproducts, such as bioethanol. [0003] Given that first-generation biofuels (derived from food sources) can only be produced in limited quantities because they compete with food production, special attention is currently being paid to second-generation biofuels (derived from non-food sources). [0004] The production proce...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12N15/04C12P19/14C12P19/02C12P7/10C12R1/885
CPCC12N9/2445C12N15/04C12P7/10C12P19/02C12P19/14C12P2201/00C12Y302/01021C12N1/145C12R2001/885C12N1/14C12N9/2437Y02E50/10C10L1/02C10L2200/0469C10L2290/26C12P7/06
Inventor F·比达德-米彻洛L·钱·奥·童A·马尔若C·科亨
Owner INST FR DU PETROLE
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