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Application of doxycycline in preparing antitumor drugs

An anti-tumor drug, doxycycline technology, applied in the direction of anti-tumor drugs, drug combinations, tetracycline active ingredients, etc., can solve the problems of endangering life safety and quality of life, low anti-tumor activity, low cure rate, etc. Low cost, wide range of sources, and the effect of inhibiting tumor metastasis

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the above-mentioned tumors generally have the ability to metastasize and invade, once the above-mentioned diseases occur, the cure rate is low, which endangers the life safety and quality of life of our people.
However, the existing anti-tumor drugs for the above-mentioned malignant tumors not only have low anti-tumor activity, but also are expensive, which are often unaffordable for patients, and some patients even give up the opportunity for treatment because they cannot afford the high price.

Method used

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  • Application of doxycycline in preparing antitumor drugs
  • Application of doxycycline in preparing antitumor drugs
  • Application of doxycycline in preparing antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Doxycycline is to the inhibitory effect of human tongue cancer, laryngeal cancer, choriocarcinoma, salivary gland tumor, human gallbladder cancer, human thymoma cell growth

[0043] (1) Cell Recovery and Culture

[0044] The cryopreserved tumor cells were taken out from the liquid nitrogen and immediately put into a 37°C water bath to thaw the cells. In a biosafety cabinet, suck the cell suspension into a centrifuge tube with an appropriate amount of medium, centrifuge at 800rpm / min for 5 minutes; discard the supernatant, suspend the cells with 1mL of medium, and suck it into a cell culture dish with an appropriate amount of medium Place the cells at 37°C, 5% CO 2 , under saturated humidity conditions. Subculture when the cells reach 80%-90% contact confluence, digest into a single cell suspension with 0.2% trypsin, and centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend the cells, and transfer the cells to 2 -Co...

Embodiment 2

[0056] Example 2: Inhibitory effect of doxycycline on cell migration of human tongue cancer, laryngeal cancer, choriocarcinoma, salivary gland tumor, human gallbladder cancer, and human thymoma

[0057] (1) Cell Recovery and Culture

[0058] The frozen cells were taken out of the liquid nitrogen and immediately put into a 37°C water bath to thaw the cells. In a biosafety cabinet, suck the cell suspension into a centrifuge tube with an appropriate amount of medium, centrifuge at 800rpm / min for 5 minutes; discard the supernatant, suspend the cells with 1mL of medium, and suck it into a cell culture dish with an appropriate amount of medium Cells were cultured at 37°C, 5% CO2, and saturated humidity. Subculture when the cells reach 80%-90% contact confluence, digest into a single cell suspension with 0.2% trypsin, and centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend the cells, and transfer the cells to Continue culturing in 2-3 Petri ...

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Abstract

The invention provides application of doxycycline in preparing antitumor drugs, wherein the tumor contains one or a combination of more of tongue cancer, laryngeal cancer, choriocarcinoma, sialaden tumor, gallbladder carcinoma and thymoma. In the application, doxycycline can be used as the drug for inhibiting tumor growth as well as the drug for inhibiting tumor metastasis. The drug can be easilyaccepted by patients, is low-cost, is extensive in source, can be easily acquired and is convenient for understanding the reaction of patients to the drug. Besides, doxycycline will change the existing market structure of tumor chemotherapeutic drugs and can become a clinical drug suitable for long-term administration and capable of effectively inhibiting tumor metastasis, invasion and relapse.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to the application of doxycycline in the preparation of antitumor drugs. Background technique [0002] Doxycycline "Doxycycline" (also known as doxycycline), belonging to the tetracycline antibiotics, is a relatively widely used antibiotic. Tetracyclines mainly act on the 30S subunit of bacteria and proteins, interfere with the combination of amino acids and ribosomal tRNA and the 30S subunit, block the combination of aminoacyl tRNA and ribosome-mRNA complex, inhibit protein synthesis, and also It can change the permeability of the cell membrane, expose important intracellular substances such as nucleotides, inhibit DNA synthesis, and achieve antibacterial effects. At the same time, it can also affect tumor cell proliferation and apoptosis, invasion and metastasis, angiogenesis, etc. process. Doxycycline is a type with stronger drug efficacy and wider application among tetracycl...

Claims

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Application Information

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IPC IPC(8): A61K31/65A61P35/00A61P35/04
CPCA61K31/65A61P35/00A61P35/04
Inventor 杨诚孙涛周红刚刘慧娟刘艳荣高晶晶
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