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Autoantibody immunochromatographic strip and test card for simultaneously detecting M-type phospholipase A2 receptor and type 1 thrombospondin 7A domain

A technology of reactive proteins and autoantibodies, which is applied in the field of clinical immunology detection, can solve problems such as complex detection methods, and achieve the effects of simple preparation method, small size and portability

Pending Publication Date: 2019-04-02
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For this reason, what the present invention is to solve is the complicated technical problem of detection method in the prior art, provides a kind of simple operation, high sensitivity, can detect PLA2R-IgG and THSD7A-IgG at the same time colloidal gold immunochromatography strip and application its reagent card

Method used

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  • Autoantibody immunochromatographic strip and test card for simultaneously detecting M-type phospholipase A2 receptor and type 1 thrombospondin 7A domain
  • Autoantibody immunochromatographic strip and test card for simultaneously detecting M-type phospholipase A2 receptor and type 1 thrombospondin 7A domain
  • Autoantibody immunochromatographic strip and test card for simultaneously detecting M-type phospholipase A2 receptor and type 1 thrombospondin 7A domain

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Embodiment Construction

[0026] Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:

[0027] In order to make the purpose, technical solution and advantages of the present invention clearer, the following will further describe in detail the embodiments of the present invention in conjunction with the accompanying drawings.

[0028] This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete and will fully convey the concept of the invention to those skilled in the art, and the present invention will only be defined by the appended claims. In the drawings, the size and relative sizes of layers and regions may be exaggerated for clarity.

[0029] In the following examples, the PLA2R, THSD7A, rabbit anti-human IgG, goat anti-rabbit IgG antibody, phosphate buffer solution, bovine ...

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Abstract

The invention relates to the field of clinical immunology detection, in particular to an autoantibody immunochromatographic strip and a test card for simultaneously detecting an M-type phospholipase A2 receptor and a type 1 thrombospondin 7A domain. The autoantibody immunochromatographic strip and the test card for simultaneously detecting the M-type phospholipase A2 receptor and the type 1 thrombospondin 7A domain comprises a bottom plate, an absorbent paper, a nitrocellulose coating film, an antibody binding pad, a sample pad, and a shell. The absorbent paper, the nitrocellulose coating film, the antibody binding pad and the sample pad are sequentially adhered to the bottom plate along the length direction of the bottom plate. The shell encloses the immunochromatographic strip. The shellfurther comprises a base and a clamping cover. The clamping cover is provided with an observing opening and a sample adding opening. The opening of the sample adding opening is located at the upper part of the sample pad. The opening of the observing opening is located at the upper side of the coating film.

Description

technical field [0001] The invention relates to the field of clinical immunology detection, in particular to an immunochromatographic strip and a detection card for simultaneous detection of M-type phospholipase A2 receptor and type 1 thrombospondin 7A domain autoantibody. Background technique [0002] The prevalence rate of kidney disease among adults in my country reaches 10.8%, and millions of people die every year due to kidney disease or cardiovascular and cerebrovascular diseases. Membranous nephropathy (MN) is a common cause of nephrotic syndrome in adults. At present, the diagnosis of membranous nephropathy mainly relies on clinical manifestations and renal puncture, which is a painful, risky, and economically burdensome process for patients. Therefore, many patients choose to refuse, miss the opportunity for early diagnosis and treatment, and delay the disease. It will be a breakthrough in the diagnosis and treatment of membranous nephropathy if other medical metho...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/573G01N33/68
Inventor 黄飚
Owner ZHEJIANG SCI-TECH UNIV
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