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Zero-valent iron-isaria fumosorosea nanoparticles, preparation method and application thereof

A technology of Rhododendron rhodozyma and nano-particles, which is applied to the methods of using spores, botanical equipment and methods, biochemical devices and methods, etc. The dosage and efficacy of C. smogum are not obvious, so as to achieve the effect of no pollution to the environment, reducing the amount of bacteria used, and improving the control effect.

Inactive Publication Date: 2019-04-02
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nano-particles prepared by the invention have high purity, strong activity, and are simple and fast. The zero-valent iron-I. It can significantly reduce the dosage of I. fumigatus, and effectively solve the problems of slow insecticidal speed and ineffective drug effect of I. fumigatus

Method used

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  • Zero-valent iron-isaria fumosorosea nanoparticles, preparation method and application thereof
  • Zero-valent iron-isaria fumosorosea nanoparticles, preparation method and application thereof
  • Zero-valent iron-isaria fumosorosea nanoparticles, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1 0

[0048] Example 1 Synthesis of Zero-Valent Iron-I.

[0049] 1. Experimental materials

[0050] I. fumigatus is I. fumigatus strain SP535, which is preserved in the Biological Control Engineering Research Center of the Ministry of Education, South China Agricultural University. It was originally isolated from the infected stinkbug. It is now preserved in the Guangdong Provincial Microbial Culture Collection Center, the address is: Guangdong Institute of Microbiology, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City; the preservation number is: GDMCC No: 60514.

[0051] (1) Identification of strains: the DNA of the purified strains was extracted with the Ezup column type fungal genome DNA extraction kit, and the ribosomal transcribed spacer gene (ITS) was amplified with a PCR instrument, and the obtained gene was listed in NCBI Compared to Cordyceps fumosorosea, a phylogenetic tree was constructed using MEGA6 using the neighbor-joining method. The result is a...

Embodiment 2 0

[0068] Example 2 The Effect of Zero-Valent Iron-Rose Fume-colored Isochora Nanoparticles

[0069] 1. Experimental method

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Abstract

The invention discloses zero-valent iron-isaria fumosorosea nanoparticles, a preparation method and an application thereof. The method comprises the following steps: inoculating a fermentation culturebroth with a spore suspension of isaria fumosorosea, and performing culturing for 48-72 hours; collecting the mycelia and putting the mycelia into sterilized distilled water and performing culturingfor 48-72 hours; collecting the conidium filtrate, adding zero-valent iron into the conidium filtrate, performing incubating for 24-96 hours to obtain the zero-valent iron-isaria fumosorosea nanoparticles. The preparation method creatively adopts the combination of zero-valent iron and isaria fumosorosea to prepare high-efficient green environmental-friendly nano-particle biological pesticide withhigh purity, strong activity, simplicity and rapidness, produced significant synergistic effects, more efficiency, longer survival time of spores in the environment, more stability, more environmental friendliness and other characteristics, has a good control effect on whitefly pests, has a very strong application potential in the biological control of whitefly pests.

Description

technical field [0001] The invention belongs to the technical field of mixed biological pesticide control. Specifically, it relates to a zero-valent iron-fume-colored I. spp. nanoparticle and its preparation method and application. Background technique [0002] With the popularity of green and environmental protection concepts, the biological method of synthesizing metal nanoparticles using biological systems has attracted much attention. The biological method is an environmentally friendly method, which is cheap, sustainable, mild in reaction conditions and stable in product. Both simple prokaryotes and complex eukaryotes can be used to synthesize nanoparticles, such as plants, bacteria and fungi. Compared with plants and bacteria, fungi are rich in metabolites and can secrete a variety of enzymes, resulting in higher quality nanoparticles. Now it has become one of the main forces of biological synthesis of nanoparticles. [0003] Cordyceps fumosoroseus is a relatively ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N59/16A01P3/00A01P7/04B82Y30/00B82Y40/00C12N1/14C12N3/00C12R1/645
CPCA01N59/16A01N63/30B82Y30/00B82Y40/00C12N1/14C12N3/00Y02A50/30
Inventor 阿里·肖卡特邱宝利吴建辉王兴民徐静杜彩莲
Owner SOUTH CHINA AGRI UNIV
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