Immunocolloidal gold test paper for rapidly detecting mycoplasma bovis and preparation method thereof

Abstract

The invention discloses an immunocolloidal gold test paper for rapidly detecting mycoplasma bovis and a preparation method thereof. The bottom layer of the test paper is a support plate, the support plate is sequentially adhered with a sample pad, a bonding pad, an NC film and an absorption pad which are closely connected. A mycoplasma bovis P33 monoclonal antibody with a colloidal gold mark is absorbed on the bonding pad, a rabbit anti-mycoplasma bovis P33 polyclonal antibody is sprayed on the NC film to take as a detection line and a sheep anti-BALB/C mouse IgG antibody is sprayed on the NCfilm to take as a quality control line, the line widths of the detection line and the quality control line are 1mm, the distance of the detection line and the quality control line is 5mm, the detection line and the quality control line are located at the center of the NC film, and the amino acid sequence of the mycoplasma bovis P33 protein is as shown such as SEQ ID NO.1. The test paper can be used for rapid detection of the mycoplasma bovis antigen to generate a result after 5-10min, the sensitivity is 5 ng/mL, the sample amount is few, cross reaction is not needed, the flexibility is high, the requirements of low cost and rapid field diagnosis can be met, and the detection efficiency can be greatly improved. The immunocolloidal gold test paper for rapidly detecting mycoplasma bovis and the preparation method thereof can develop important effects in the prevention and control of bovine mycoplasmosis and have potential promotion and application values.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to an immune colloidal gold test paper for rapidly detecting mycoplasma bovis and a preparation method thereof. Background technique [0002] Mycoplasma bovis (Mycoplasma bovis, Mb) is a kind of simple and extremely small prokaryotic microorganisms without cell walls, between bacteria and viruses, capable of self-replication and independent living, which can cause bovine pneumonia, mastitis, arthritis and many other diseases. This disease has caused huge economic losses and seriously threatened the healthy development of my country's cattle industry. [0003] At present, the detection of Mycoplasma bovis in the prior art mainly includes methods such as pathogen isolation and identification, polymerase chain reaction (PCR), fluorescent quantitative PCR technology, enzyme-linked immunosorbent assay (ELISA), but these methods are all subject to clinical application. Lar...

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Problems solved by technology

[0005] For above-mentioned deficiencies in the prior art, the object of the present invention is to provide a kind of immunocolloidal gold test paper and preparation method thereof of quick detection Mycoplasma bovis, solve the existing Mycoplasma bovis detection method to have complicated operation, long detection time, can not realize on-the-spot rapid detection and high cost

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