Double two-position four-way valve-based multi-dimensional liquid chromatography separation system

Abstract

The invention provides a multi-dimensional liquid chromatography separation system. The system comprises a high-performance liquid chromatography gradient pump A, a high-efficiency liquid chromatography gradient pump B, a high-performance liquid chromatography diluting pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a double-two-position four-way valve and a connecting pipeline; and switching between a previous one-dimensional separation state and a next one-dimensional separation state is realized through switching of the double-two-position four-way valve, and chromatography separation of three dimensions or above is realized. A chromatography separation column of each dimension is selected through the liquid chromatography separation column array; and on the basis of the same gradient elution system and the same detector, full online monitoring and control of multi-dimensional chromatography separation are realized, and the cleaning degree of enrichment columns and separation columns is controllable. The separationof all dimensions is connected through the enrichment columns, and the diluting pump is used for assisting in enrichment or trapping of compounds. Different chromatography stationary phase and mobilephase combinations are selected, so that efficient separation of monomeric compounds in a complex system sample with high separation difficulty is realized.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multidimensional liquid chromatography separation system. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize two or more chromatographic columns with different separation mechanis...

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Problems solved by technology

However, the system is based on a 2-position 10-way valve. Due to the asymmetrical flow path of the 2-position 10-way valve, the application performance of the system is affected to a certain extent.

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