Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A novel electrochemical biosensor for detection of α2,6 sialylated glycans

A biosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as expensive, achieve the effect of less detection steps, promote development, and improve biocompatibility

Active Publication Date: 2021-09-03
CHONGQING MEDICAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods require expensive equipment and materials, and the tested samples must undergo complex pretreatment to eliminate other interferences. In addition, they must be analyzed by technicians in specialized laboratories

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A novel electrochemical biosensor for detection of α2,6 sialylated glycans
  • A novel electrochemical biosensor for detection of α2,6 sialylated glycans
  • A novel electrochemical biosensor for detection of α2,6 sialylated glycans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Step 1. Dispense 1mL of 2.5mg mL -1 The solution of carboxylated single-walled carbon nanohorns (c-SWCNHs) was sonicated for 5 min; then, 5 mL of potassium chloroplatinate (K 2 PtCl 4 ) and 6 mL of 0.1 mM methylene blue (MB) were heated and stirred at 90° C. for 5 hours. Finally, the mixture was centrifuged at 10,000 rpm for 5 minutes, washed three times, dissolved in 1 mL of ultrapure water, and finally dried in a vacuum oven for use.

[0038] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0039] Step 3. Add 10 μL of electrode modification material gold nanorods-streptavidin (AuNRs-SA) dropwise on the electrode surface, and dry at room temperature;

[0040] Step 4. Dissolve 8 μL of bone-synthetic black wood protein (bio-SNA) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention successfully developed a specific hypersensitivity based on novel gold nanorod-streptavidin (AuNPs-SA) composites and carboxylated single-walled carbon nanohorns-sulfur-doped platinum (c-SWCNHs / S-PtNC) Sandwich electrochemical immunosensor for the detection of α2,6 sialylated glycans (α2,6‑sial‑Gs) in human serum. Gold nanorods-streptavidin (AuNPs-SA) can not only increase the conductivity of the electrode and enhance the transfer of electrons, but also provide biotin-labeled black elderin (bio -SNA) provides the active site. In addition, c‑SWCNHs / S‑PtNC has a positive effect on H 2 o 2 The reduction of has excellent catalytic performance, and Pt‑NH 2 and ‑NH‑CO‑ for effective capture of triaminophenylboronic acid (M‑APBA). The advantage of the present invention is that the linear range is wide. The invention has high sensitivity, strong specificity, rapid detection, and good reusability, and the invention can be used to measure α2,6-sial-Gs content in human serum, and has great potential in clinical detection.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical immunosensor for quantitative detection of α2,6 sialylated glycans in clinical practice, especially based on gold nanorod-streptavidin nanoparticle composite material and carboxylated single-walled carbon The nanohorn-sulfur-doped platinum nanocomposite material is used as a biosensor prepared as a signal probe for detecting α2, 6 sialoglycan, belonging to the field of electrochemical detection. Background technique: [0002] α2,6-sialylated glycan (α2,6-sial-Gs) is a broad-spectrum tumor marker, which is closely related to malignant behaviors such as tumor proliferation, metastasis, spread and tumor antigenicity. During the apoptosis process of cancer cells, α2,6-sial-Gs is shed under the hydrolysis of glycosidase and released into the blood circulation, so that the level of α2,6-sial-Gs in serum is significantly increased. Therefore, the detection of α2,6-sial-Gs ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/531
CPCG01N27/26G01N27/30G01N27/3278G01N33/531
Inventor 于超何俊琳李佳
Owner CHONGQING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com