Preparation method and application of metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex
A technology of earthworm protein and oxide, which is applied in the direction of drug combination, hydrolyzed protein components, peptide/protein components, etc., can solve the problems of high economic cost, large consumption, and difficulty in commercial production, so as to promote recovery and reduce oxidation loss effect
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[0024] The embodiment of the present embodiment is a method for preparing metal oxide nano-Bletilla striata dilong protein complex, comprising the following steps:
[0025] S1, preparation of earthworm stress protein:
[0026] Wash the fresh earthworm (Essium chinensis) with 0.9% NaCl solution for several times to remove the dirt on the body surface, and place the cleaned earthworm in physiological saline solution with an air pump continuous oxygen supply system at 25°C Let it spit out the sediment in the body for 4 hours; remove the surface moisture with absorbent paper and transfer it to a beaker; add 10% sucrose of the total weight of earthworm and quickly stir it evenly with a glass rod, wait for 10 minutes and collect the water in the beaker The yellow-green liquid is the earthworm stress protein, add 1X anti-protein degradation enzyme, and then add NaN with a concentration of 0.02% 3 Obtain the earthworm stress protein liquid as a preservative, and store it for future u...
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