Porcine immortalized alveolar macrophage cell line for expressing porcine reproductive and respiratory syndrome virus acceptor CD163 and application thereof

A technology for alveolar macrophages and respiratory syndrome, applied to viruses/bacteriophages, genetically modified cells, cells modified by introducing foreign genetic material, etc., can solve problems such as inability to express CD163, inability to support PRRSV virus replication, etc.

Inactive Publication Date: 2019-03-26
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies have shown that the application of SV40 Large T antigen can immortalize primary PAM cells, but CD163 cannot be expressed after immortalization, so it cannot support the replication of PRRSV virus in cells, and PRRSV must be mediated by reintroducing CD163 receptors Viral infection

Method used

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  • Porcine immortalized alveolar macrophage cell line for expressing porcine reproductive and respiratory syndrome virus acceptor CD163 and application thereof
  • Porcine immortalized alveolar macrophage cell line for expressing porcine reproductive and respiratory syndrome virus acceptor CD163 and application thereof
  • Porcine immortalized alveolar macrophage cell line for expressing porcine reproductive and respiratory syndrome virus acceptor CD163 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Establishment of Immortalized Porcine Alveolar Macrophage (PAM) Cell Line Highly Expressing Porcine Reproductive Disorder and Respiratory Syndrome Virus (PRRSV) Receptor CD163

[0024] 1 Materials and methods

[0025] 1.1 Materials

[0026] 1.1.1 Experimental animals

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Abstract

The invention discloses a porcine immortalized alveolar macrophage cell line for expressing a porcine reproductive and respiratory syndrome virus, PRRSV, acceptor CD163. The cell line is named as PAM-Tang-20 which is preserved in CGMCC, the preservation number of culture of which being CGMCC No. 13591. Experiments verify that the cell line established by the invention can express the CD163 acceptor highly, is a susceptible cell of PRRSV, and can be widely applied to proliferation of PRRSV, separation of PRRSV clinical strains, vaccine production, PRRSV related basic researches and the like. The cell line for provides an effective technical means for in vitro culture of the PRRSV.

Description

technical field [0001] The present invention relates to a cell line that can be used for culturing porcine reproductive disorder and respiratory syndrome virus in vitro and its application, in particular to a porcine immortalized alveolar macrophage capable of highly expressing porcine reproductive disorder and respiratory syndrome virus receptor CD163 A cell line and its application, the invention belongs to the field of biotechnology. Background technique [0002] Porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV) is an important pathogen that endangers the pig industry all over the world. Especially after the emergence of highly pathogenic PRRSV, the pig industry in my country is facing more serious problems. [0003] PRRSV is a single-stranded positive-strand RAN virus with a genome of about 15 kb belonging to the genus Arterivirus. According to the latest virus classification, the Arterivirus genus also incl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N7/00C12R1/93
CPCC12N5/0645C12N7/00C12N2510/04C12N2770/10051
Inventor 蔡雪辉汤艳东刘永刚王刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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