MacConkey improved culture medium with improved separating effect

A technology of culture medium and bile salts, applied in the field of culture medium, can solve problems such as troublesome detection of separation results, accuracy and validity, influence on the growth of target isolates, false negative results, etc., to avoid false negative results and to achieve scientific and reliable results. , judge the simple effect

Inactive Publication Date: 2019-03-22
徐宙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing MacConkey agar medium can meet the most basic nutritional and separation requirements, that is, it can ensure the color separation of Gram-negative enterobacteria that ferment lactose. However, there are many types of miscellaneous bacteria, especially mixed bacteria. When there are many Gram-positive bacteria in the medium, it will cause sepa...

Method used

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  • MacConkey improved culture medium with improved separating effect
  • MacConkey improved culture medium with improved separating effect
  • MacConkey improved culture medium with improved separating effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A MacConkey-improved medium for improving the separation effect: in every 1000mL medium, tryptone 15g, soytone 5g, pig bile salt 5g, sodium wolfberry 2g, sodium chloride 5g, lactose 10g, neutral red 0.075g , Agar 12g; pH=7.4±0.2.

[0019] The preparation steps of the above-mentioned medium are as follows: mix the above-mentioned weight of tryptone, soytone, lactose, pig bile salt, sodium wolfberry, sodium chloride, neutral red, and agar, add purified water to constant volume, heat to dissolve, and adjust the pH value to 7.4±0.2, sterilized at 121°C for 15 minutes, cooled to 50°C, poured into the plate, condensed to obtain the finished medium, and set aside.

Embodiment 2

[0021] MacConkey’s improved medium to improve the separation effect: in every 1000mL medium, tryptone 15g, soytone 5g, porcine bile salt 5g, sodium wolfberry 1.5g, sodium chloride 5g, lactose 10g, neutral red 0.075g, Agar 12g; pH=7.4±0.2.

[0022] The preparation method is the same as in Example 1.

Embodiment 3

[0024] MacConkey’s improved medium to improve the separation effect: in every 1000mL medium, tryptone 16g, soytone 4g, porcine bile salt 4g, sodium wolfberry 2.5g, sodium chloride 4g, lactose 10g, neutral red 0.075g, Agar 14g; pH=7.4±0.2.

[0025] The preparation method is the same as in Example 1.

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Abstract

The invention relates to the technical field of culture media and in particular relates to a MacConkey improved culture medium with improved separating effect. The improved culture medium is preparedfrom a nitrogen source, cholate, sodium citrate, sodium chloride, lactose, neutral red and agar, wherein a pH is 7.4+/-0.2. The improved culture medium is capable of remarkably improving the separating effect, and guaranteeing the excellent effect of realizing target bacteria separation in the presence of multiple gram-positive bacteria or more increment of the gram-positive bacteria.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a MacConkey-improved medium for improving the separation effect. Background technique [0002] MacConkey medium is an isolation and identification medium with moderate selectivity and slightly strong antibacterial ability, mainly inhibiting Gram-positive bacteria. The existing MacConkey agar medium can meet the most basic nutritional and separation requirements, that is, it can ensure the color separation of Gram-negative enterobacteria that ferment lactose. However, there are many types of miscellaneous bacteria, especially mixed bacteria. When there are many Gram-positive bacteria in the medium, it will cause separation difficulties, especially in clinical testing, and even excessive growth of miscellaneous bacteria will have a great impact on the growth of target isolates, and further isolation and detection will cause false negative results. It is more terrible than f...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/20C12Q1/045
Inventor 徐宙
Owner 徐宙
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