Method used for automatically extracting target cell mitochondrion

Abstract

The invention discloses a method used for automatically extracting a target cell mitochondrion and belongs to micro nano robot assisted control systems, and automatic cell pose control and two-dimensional cell biopsy operation are realized. The method comprises the steps that a CCD camera connected with a fluorescence microscope is used for collecting a cell image, and the position and the pose angle of the to-be-extracted target cell mitochondrion are recognized with an image processing algorithm; accurate control of the cell position and the pose angle is realized respectively through different operation modes of optical tweezers; a pump is externally connected to a micro-fluidic chip to add negative pressure, so that the target cell is firmly fixed in the passage of the micro-fluidic chip to keep the pose; the sharp end of a glass micro pipe moves to the nearby of the to-be-extracted target cell mitochondrion with a visual feedback control strategy; a target cell is extracted from the to-be-extracted target cell mitochondrion through the negative pressure applied by the pump externally connected to the glass micro pipe.

Description

technical field [0001] The invention belongs to a micro-nano robot auxiliary control system, in particular to a method for automatically extracting target cell mitochondria. Background technique [0002] Bioanalysis at the single-cell level can capture cell heterogeneity and has received extensive attention in recent years. As a single-cell biopsy technique, cell biopsy achieves disease diagnosis and treatment by extracting and analyzing tissues or organelles at the subcellular level. Therefore, it is imperative to develop highly precise micromanipulation tools capable of manipulating and extracting organelles and intracellular components to facilitate the rapid development of new single-cell-based medical therapies, thereby facilitating in-depth insights into cellular dynamics, cellular component functions, and disease mechanisms. understand. [0003] In the past decades, a variety of micro / nano manipulation tools for intracellular level manipulations have been developed,...

AI Technical Summary

Problems solved by technology

These operating systems and tools are generally implemented by open-loop manual methods, which require high operational proficiency and require a lot of time for operational training, poor repeatability of experiments, and low operational accuracy.

Cell biopsy manipulation tasks, such as locating the position of the microtubule tip relative to the cell, locating the glass microtubule at a specific location within the cell after piercing the cell, and extracting tiny amounts of tissue from the cell, are tedious and time-consuming

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