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Extraction and preparation method of small molecular fish skin collagen peptide

A technology of fish skin collagen and small molecules, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of wide molecular weight distribution, high price of protease, and limited application range, etc., and achieve good application Value and market potential, the effect of increasing the speed of enzymatic hydrolysis, and being easy to produce on an industrial scale

Inactive Publication Date: 2019-03-19
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of protease, if the extraction process is not properly designed, not only a large amount of protease needs to be added, but also the enzymatic hydrolysis time is long, resulting in high production costs and wide molecular weight distribution of the product
On the other hand, the small molecule collagen peptides prepared from fish skin are dark in color and have a fishy smell, which limits the scope of application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for extracting and preparing small molecule fish skin collagen peptides, specifically comprising the following steps:

[0036] S1. Raw material preparation: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0037] S2. Degreasing treatment: add sodium hydroxide and water to the chopped fish skin, stir for 20 minutes, let it stand for 4 hours, filter the water, carry out degreasing treatment on the fish skin, rinse the fish skin twice after degreasing; The addition of water is 1% of the quality of fish skin, and the addition of water is 2 times of the quality of fish skin.

[0038] S3. Enzymatic hydrolysis pretreatment: add deionized water twice the weight of the minced fish skin to the degreased minced fish skin, beat and mix to obtain a slurry;

[0039] S4. Enzymolysis treatment: first add 0.1% flavor protease by weight of the slurry, adjust the pH value to 6.5, heat the slurry ...

Embodiment 2

[0049] A method for extracting and preparing small molecule fish skin collagen peptides, specifically comprising the following steps:

[0050] S1. Raw material preparation: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0051] S2. Degreasing treatment: Add sodium hydroxide and water to the chopped fish skin, stir for 20 minutes, let it stand for 4.5 hours, filter off the water, carry out degreasing treatment on the fish skin, rinse the fish skin twice after degreasing; The added amount of sodium is 1.2% of the mass of fish skin, and the added amount of water is 2.5 times of the mass of fish skin.

[0052] S3. Enzymatic hydrolysis pretreatment: add 2.5 times the weight of deionized fish skin to the defatted minced fish skin, beat and mix to obtain a slurry;

[0053] S4. Enzymolysis treatment: first add 0.12% of the weight of the slurry flavor protease, adjust the pH value to 6.5, heat the slu...

Embodiment 3

[0063] A method for extracting and preparing small molecule fish skin collagen peptides, specifically comprising the following steps:

[0064] S1. Raw material preparation: take fresh fish to collect fish skin, wash the fish skin, drain the water on the surface of the fish skin, and chop the fish skin;

[0065] S2. Degreasing treatment: add sodium hydroxide and water to the chopped fish skin, stir for 25 minutes, let it stand for 5 hours, filter the water, carry out degreasing treatment on the fish skin, rinse the fish skin 3 times after degreasing; The addition of water is 1.5% of the quality of fish skin, and the addition of water is 3 times of the quality of fish skin.

[0066] S3, enzymatic hydrolysis pretreatment: add 5 times the weight of deionized fish skin to the defatted minced fish skin, beat and mix to obtain a slurry;

[0067] S4. Enzymolysis treatment: first add 0.15% of the weight of the slurry flavor protease, adjust the pH to 7.0, heat the slurry to 60°C, and ...

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Abstract

The invention discloses an extraction and preparation method of small molecular fish skin collagen peptide. The extraction and preparation method comprises the following steps: preparing raw materials, carrying out enzymolysis pre-treatment, carrying out enzymolysis treatment and the like. According to the extraction and preparation method of the small molecular fish skin collagen peptide, provided by the invention, fish skin is firstly subjected to degreasing treatment and then is subjected to the enzymolysis treatment; and in an enzymolysis treatment process, various types of different proteinases are used for carrying out enzymolysis on the fish skin, so that the enzymolysis speed can be improved, the dosage of the proteinase also can be reduced, and the yield of the small molecular fish skin collagen peptide can be effectively improved. According to the extraction method provided by the invention, modified activated carbon is used for de-coloring, the adsorption performance is excellent and enzymolysis liquid can be uniformly distributed; good de-coloring and deodorization effects can be realized under the conditions of small dosage and short time and effective components are not lost; and the extraction method has the advantages of low investment, simplicity in operation, good protein degradation effect and high yield of the fish skin collagen peptide, the industrial large-scale production is easy to realize and the extraction method has good application value and market potential.

Description

technical field [0001] The invention relates to the technical field of extracting collagen peptides, in particular to a method for extracting and preparing small molecule fish skin collagen peptides. Background technique [0002] Collagen is a natural biopolymer synthesized by animal cells. As an important component of the extracellular matrix, it exists in almost all tissues, accounting for about 30% of the total animal protein. Collagen has a high ability to resist extension, making skin and other connective tissue Tissues can maintain a certain structure and have special mechanical properties to support and protect cells, tissues and body functions, which is critical to the structure and performance of cells and tissues. [0003] Collagen peptide refers to a low-molecular-weight biologically active skin with special functions obtained from collagen tissue through deep hydrolysis. Compared with collagen, collagen peptide is more easily absorbed by the human body, and the a...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 洪鹏志刘唤明周春霞邓楚津杨萍
Owner GUANGDONG OCEAN UNIVERSITY
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