A transcription factor gene regulating plant flavonoid synthesis and its application

A technology of transcription factors and flavonoids, applied in plant genetic improvement, plant products, genetic engineering, etc., can solve the problems of insufficient bZIP transcription factor genes, difficulty in increasing flavonoid content in large quantities, etc., and achieve the effect of increasing content

Active Publication Date: 2021-09-17
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0004] Aiming at the problem of insufficient bZIP transcription factor genes in the biosynthesis pathway of flavonoids in the existing research, and it is difficult to increase the content of flavonoids in a large amount, the present invention provides a transcription factor gene for regulating the synthesis of flavonoids in plants and its application. The gene is a New gene, the bZIP family transcription factors that regulate the synthesis of flavonoids (anthocyanidins and proanthocyanidins) have not been found in Medicago truncatula. Therefore, it is necessary to study the bZIP transcription factors that regulate the biosynthesis of flavonoids in Medicago truncatula

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  • A transcription factor gene regulating plant flavonoid synthesis and its application
  • A transcription factor gene regulating plant flavonoid synthesis and its application
  • A transcription factor gene regulating plant flavonoid synthesis and its application

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0019] 1. Main experimental materials and methods

[0020] 1.1 CTAB method to extract the genomic DNA of Medicago truncatula

[0021] 1) Take about 100 mg of Medicago truncatula tissue material and place it in a 2 mL centrifuge tube together with steel balls; add liquid nitrogen and shake continuously until the tissue material is fully ground into powder.

[0022] 2) Add 500 μL of LCTAB extract solution (preheated at 65°C), and vortex to mix well.

[0023] 3) ...

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Abstract

The invention discloses a transcription factor gene that regulates the synthesis of plant flavonoids. The transcription factor gene is the MtbZIP10 gene identified from the leguminous plant Medicago truncatula, and its gene sequence is shown in SEQ ID NO.1. Moreover, the present invention discloses the function of the gene and its application. The advantage of the present invention is that the transcription factor gene that can positively regulate the synthesis of flavonoid compounds is cloned from the leguminous plant Medicago truncatula, and its function is systematically identified, and it is found that the gene can activate the biosynthesis pathway of flavonoids Multiple genes that increase the content of flavonoid compounds.

Description

technical field [0001] The invention belongs to the technical field of gene cloning, in particular to a transcription factor gene for regulating the synthesis of plant flavonoids and its use. Background technique [0002] Flavonoids are a large class of important secondary metabolite products widely distributed in the roots, stems, leaves, flowers, fruits and seed coats of plants, including flavonoids, isoflavones, flavonols, anthocyanins and procyanidins, etc. . Among them, anthocyanins have the functions of protecting plants from biological damage and attracting insect pollination. Anthocyanins can also respond to biotic and abiotic stresses, scavenge oxygen free radicals, and protect plants from high-density light damage. At the same time, anthocyanins have certain health care functions in anti-cancer, anti-disease and anti-oxidation. Proanthocyanidins, another type of flavonoids, not only have the effect of protecting plants from herbivores and pathogenic bacteria, but...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00A01H6/54
CPCC12N9/1085C12N15/825
Inventor 庞永珍宋雪薇狄少康
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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