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A dual-signal amplifying biosensor assembly method and its application

A biosensor, dual-signal amplification technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of limited photoelectrochemical applications, low photoelectric conversion efficiency, etc., and achieve the effect of enhancing photoelectric signals

Active Publication Date: 2020-09-15
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many studies have shown that WS 2 has very low photoelectric conversion efficiency, thus limiting its photoelectrochemical application
In addition, the construction of topography and surface structure is also a great challenge

Method used

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  • A dual-signal amplifying biosensor assembly method and its application
  • A dual-signal amplifying biosensor assembly method and its application
  • A dual-signal amplifying biosensor assembly method and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] WS 2 The preparation method of nanowire array material, comprises the following steps: in 200mL beaker, add 1.6493gNa 2 WO 4 2H 2 O was added with 40 mL of deionized water and stirred for 30 min. 3M HCl was added dropwise to adjust the pH of the solution to 1.2, at which point the solution changed from colorless to light yellow. 1.765gH 2 C 2 o 4 Add to the above mixed solution, stir until dissolved, then dilute to 100mL to obtain H 2 WO 4 precursor. Take 40mLH 2 WO 4 Add 2g (NH 4 ) 2 SO 4 And 0.411g thiourea, stirred until dissolved, transferred to the autoclave. A 2cm×4cm titanium mesh was treated several times with concentrated hydrochloric acid, ethanol, and deionized water successively, and then put into a reaction kettle, and reacted at 180°C for 16 hours. After the reaction, the titanium mesh was taken out, washed several times with deionized water, and dried at 60° C. for 12 hours. The obtained sample was transferred to a tube furnace, and fired ...

Embodiment 2

[0034] A method for assembling a dual-signal amplified biosensor of the present invention comprises the following steps: add 50 μL, 0.4 mg / mL polypeptide solution and 50 μL, 0.4 mg / mL glucose oxidase solution to 2 mL of AuNPs solution, and place on a shaker to avoid light Shake for 12 hours. The obtained probe was centrifuged at 12000 rpm at 4°C for 10 min, dispersed in 2 mL of deionized water, and stored in a refrigerator at 4°C. Will 0.5cm×0.5cmWS 2 Nanowire arrays were immersed in 5 μL of aptamer solution and incubated for 4 hours. The electrodes were blocked with 1% bovine serum albumin for 30 min, and the obtained electrodes were rinsed with deionized water. Then, 0.5, 1, 2, 5, 8, 10 ng / mL of HER2 was added to the electrode, incubated in a refrigerator at 4°C for 2 hours, and rinsed with deionized water. Finally, 40 μL of labeled gold nanoprobes were added to the electrode and incubated for 2 hours. After the assembled electrodes were incubated in 10 mM glucose soluti...

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Abstract

The invention discloses an assembling method and application of a double-signal-amplification biosensor and belongs to the field of nanometer new materials. The assembling method comprises the steps of preparing a gold nanoprobe and assembling a sandwich type biosensor; the assembled double-signal-amplification biosensor is applied to HER2 (human epidermal growth factor receptor 2) detection. Theassembling method of the double-signal-amplification biosensor is simple and easy to operate; the assembled double-signal-amplification biosensor can strengthen photoelectronic signals; surface plasmaresonant AuNPs can produce collective oscillation of free electrons, so that hot electrons can be transferred directly from the AuNps to the transfer belt of WS2 to further strengthen the photoelectronic signals and accordingly to achieve double-signal amplification HER2 detection.

Description

technical field [0001] The invention belongs to the field of new nanometer materials, and in particular relates to an assembly method and application of a dual-signal amplified biosensor. Background technique [0002] Molybdenum disulfide (WS 2 ) have the advantages of large surface area, excellent carrier fluidity, high active site density, wide light absorption and easy functionalization, etc., and are widely used in the fields of hydrogen production, optical sensing, bioimaging and cancer therapy. . However, many studies have shown that WS 2 It has very low photoelectric conversion efficiency, thus limiting its photoelectrochemical application. In addition, the construction of topography and surface structure is also a great challenge. [0003] Therefore, introducing a depleted electron donor (H 2 o 2 ) and localized surface plasmon resonance noble metal nanoparticles (Au NPs), which can improve the photoelectric characteristics and double the photoelectric signal. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/416B82Y5/00B82Y30/00B82Y40/00
CPCB82Y5/00B82Y30/00B82Y40/00G01N27/305G01N27/3278G01N27/416
Inventor 渠凤丽郭晓茜
Owner QUFU NORMAL UNIV
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