Controllable tomato genic male sterile line established by using S1PIF3 gene and establishing method thereof, and application

Abstract

The invention discloses a method and application for establishing a controllable tomato genic male sterile line by using an S1PIF3 gene. On the basis of isolation and identification of a tomato phytochrome interaction factor S1PIF3 which controls the stamen development, a homozygous mutated strain for cultivating a tomato SlPIF3 gene is obtained by using a gene-directed editing technique or a mutagenesis technique, and the vegetative growth and pistil development of the mutated plant are not affected, but pollen abortion shows male infertility. Auxin is applied to a sterile plant from an external source to restore part of the pollen fertility and form seed-producible fruits; and harvested seeds are planted into male-sterile plants without spraying the auxin. Through the above ways, a restorable genic male sterile line can be multiplied and formed, which solves the problem that the current use of a test cross method to preserve the genic recessive sterile line requires removal of half of fertile individual plants. The technology simplifies the cultivation process of tomato male sterile lines, can improve the purity of hybrids and is convenient for breeding of excellent hybrid varieties of tomatoes.

Description

technical field [0001] The invention relates to the technical field of crop molecular breeding, in particular to a method for creating a controllable nuclear male sterile line of tomato by using the SlPIF3 gene and its creation method and application. technical background [0002] There are mainly three types of crop sterile systems currently available in production, one is cytoplasmic male sterile, sometimes also called cytoplasmic-nuclear interaction sterile line. At present, it is widely used in hybrid breeding of rice, rape and other crops, but it has difficulties in the selection of restorer lines, cumbersome transfer, and a relatively long breeding cycle; the cytoplasm is single, and is easily affected by temperature, and the instability of fertility leads to possible Fertility and infertility transformation and many other issues. The second category is nuclear male sterility. The combination is free, and it is easy to have a strong dominant combination, especially t...

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Problems solved by technology

Mutation of a gene encoding bHLH transcription factor in tomato produces male sterile mutant ms10 35 (Jeong et al., 2014), the rice gene bHLH142 knockout plants showed pollen abortion, although many studies have pointed out that the bHLH family genes may be involved in the regulation of pollen development, and studies in Arabidopsis have shown that PIF3 is involved Photoperiod response, photomorphogenesis, abiotic resistance, hormone signal transduction regulation, and pollen development process also involves hormone signal transduction and other regulatory pathways. No report

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