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A method for preparing stevia polyphenols by composite chitosan flocculation method

A technology of chitosan and quaternary ammonium salt chitosan is applied in the directions of medical preparations, pharmaceutical formulations, sugar derivatives, etc. containing active ingredients, to achieve the effects of short flocculation time, low temperature and high flocculation efficiency

Active Publication Date: 2021-07-27
DONGTAI HIRYE BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The above method of extracting polyphenols from Eucommia leaf water extract, Shuanghuanglian water extract, honeysuckle, Qinggan Lidan oral liquid raw medicine water extract only focuses on extracting polyphenols

Method used

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  • A method for preparing stevia polyphenols by composite chitosan flocculation method
  • A method for preparing stevia polyphenols by composite chitosan flocculation method
  • A method for preparing stevia polyphenols by composite chitosan flocculation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Stevia leaf extraction:

[0051] Stevia leaves were extracted according to the ratio of water to dry leaves of stevia leaves at 20BV, the extraction temperature was 50°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0052] (2) Composite chitosan flocculation

[0053] After the stevia leaf water extract is adjusted to pH4.5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 10% of composite chitosan quality, surplus is shell Polycan; the molecular weight of chitosan is 100KDa, and the degree of deacetylation is 80%), and the addition amount is 0.15mL per gram of dry leaves. At 20°C, stir at 30 rpm for 5 minutes, and let stand for 40 minutes to flocculate and remove impurities; then, separate the flocculation supernatant through plate and frame filtration; detect and calculate the retention rate of steviol glycosides...

Embodiment 2

[0071] (1) Stevia leaf extraction:

[0072] Stevia leaves were extracted according to the ratio of water to dry stevia leaves of 15BV, the extraction temperature was 60°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0073] (2) Composite chitosan flocculation:

[0074] After the stevia leaf water extract is adjusted to pH5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 15% of composite chitosan quality, surplus is chitosan ; the molecular weight of chitosan is 50KDa, the deacetylation degree is 70%), and the addition amount is 0.1mL per gram of dry leaves; at 40°C, stir at 30rpm for 5min, and let stand for 35min to flocculate and remove impurities; then, separate through plate and frame filtration The flocculation supernatant was obtained; the retention rate of steviol glycosides after flocculation was detected and calculat...

Embodiment 3

[0078] (1) Stevia leaf extraction:

[0079] The stevia leaves were extracted according to the ratio of water to dry stevia leaves of 20BV, the extraction temperature was 60°C, the extraction time was 3h, and the water extract of stevia leaves was obtained by filtration;

[0080] (2) Composite chitosan flocculation:

[0081] After the stevia leaf water extract is adjusted to pH4.5 with the acetic acid aqueous solution of 1mol / L, add 1% composite chitosan acetic acid aqueous solution (quaternary ammonium salt chitosan accounts for 15% of composite chitosan quality, surplus is shell Polycan; Chitosan molecular weight is 80KDa, deacetylation degree is 80%), the addition amount is 0.12mL per gram of dry leaves; Stir at 30rpm for 5min at 40°C, let stand for 50min to flocculate and remove impurities; then, through the plate frame The flocculation supernatant was obtained by filtration and separation; the retention rate of steviol glycosides after flocculation was detected and calcul...

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Abstract

The invention discloses a method for preparing stevia polyphenols by using a composite chitosan flocculation method, and belongs to the technical field of plant extraction. In the present invention, the water extract of stevia leaf is flocculated and removed with composite chitosan at room temperature, and the supernatant is adsorbed with a macroporous polar resin; the effluent is used to further extract steviol glycosides; the resin is eluted with alcohol aqueous solutions of different concentrations , after elution with low-concentration alcohol aqueous solution, the obtained analysis solution is combined with the effluent before analysis to obtain a combined solution, the total phenol content in the combined solution is less than 0.5% (on a dry basis), and the combined solution is retained for extracting steviol glycosides. The eluate after elution with high-concentration alcohol aqueous solution is dried to obtain stevia polyphenols mainly composed of isochlorogenic acid. The invention realizes the simultaneous production of stevia polyphenols with high isochlorogenic acid on the basis of the original steviol glycoside production process, realizes the comprehensive utilization of stevia, and improves the economic benefits of enterprises.

Description

technical field [0001] The invention relates to a method for preparing stevia polyphenols by a composite chitosan flocculation method, belonging to the technical field of plant extraction. Background technique [0002] Stevia is a perennial herb that originated in South America and is widely planted in Central America, South Korea, Paraguay and other places. The content of inulin is 6%-12%. The steviol glycoside extracted from it is a low-calorie, high-sweet natural sweetener, which is widely used in food and pharmaceutical industries. Its sweetness is 150-300 times that of sucrose . In addition to the well-known steviol glycosides, alcohol, water or alcohol-water extracts of Stevia rebaudiana also contain polyphenols, flavonoids, quinic acid and caffeic acid and their derivatives, amino acids and fatty acids and their derivatives, and other monomers that need to be removed. Ning, tannins, pigments and pectin and other impurities. [0003] In stevia extract, steviol glyco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256A61K36/28
CPCA61K36/28A61K2236/331A61K2236/53A61K2236/55
Inventor 夏咏梅朱理平何东生陈科材周卓愉樊晔刘湘
Owner DONGTAI HIRYE BIOTECH CO LTD
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