Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for evaluating influence on energy metabolism of living body caused by electromagnetic radiation by using MFC

A technology of energy metabolism and electromagnetic radiation, applied in microorganism-based methods, biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of low experimental reproducibility, inconsistent experimental methods and experimental standards, and reliable experimental results. questions such as reliability and credibility, to achieve the effect of good repeatability, simple operation, and extended application

Active Publication Date: 2019-03-01
FUJIAN ACAD OF MEDICAL SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no biomarkers that can be used to indicate the effect of electromagnetic fields on the energy metabolism of organisms have been found
The main reason is that the above studies need to lyse the cells, so the experimental results cannot reflect the influence of electromagnetic fields on the energy metabolism of organisms in real time.
In addition, due to the inconsistency of experimental conditions (such as electromagnetic field strength), experimental methods, and experimental standards, the reproducibility of some experiments is low, leading to doubts about the reliability and credibility of experimental results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating influence on energy metabolism of living body caused by electromagnetic radiation by using MFC
  • Method for evaluating influence on energy metabolism of living body caused by electromagnetic radiation by using MFC
  • Method for evaluating influence on energy metabolism of living body caused by electromagnetic radiation by using MFC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The embodiments described below are exemplary and are intended to explain the present invention, but should not be construed as limiting the present invention.

[0030] Example 1:

[0031] (1) Strain cultivation: the electrogenic microorganism Shewanella Oneida ( Shewane1la oneidensis MR-1, ATCC700550) were streaked and inoculated in solid LB medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, agar powder 15 g, pH adjusted to 7.0), cultured at 30°C After 24 hours, a single colony was picked and placed in liquid LB medium, 30°C, 180 rpm, and cultured for 12 hours.

[0032] (2) Preparation of MFC inoculum solution: Centrifuge the bacterial solution in (1) at 6000 r / min at 4°C for 2 min, remove the supernatant under anaerobic conditions, and obtain bacterial cells, use PBS buffer solution, the composition is ( / L): Na 2 HPO 4 12H 2 O 22.2 g; NaH 2 PO 4 2H 2 O5.93 g; KCl 0.13 g, pH7.0, washed three times, and resuspended in 0.5 mL to obtain MFC inoculum.

...

Embodiment 2

[0039] (1) Strain cultivation: the electrogenic microorganism Geobacter sulfur-reducing ( Geobacter sulfur reducers PCA, DSMZ12127) were inoculated in liquid NBAF medium (fumaric acid 4.64 g / L, NaHCO 3 2.50 g / L, NH 4 Cl0.25 g / L, NaH 2 PO 4 2H 2 O 0.68 g / L, KCl 0.10 g / L, vitamin mother solution 10 ml / L, trace element mother solution 10 ml / L, sodium acetate 0.85 g / L, pH adjusted to 7.0), the inoculation amount of the bacterial solution was 10% of the inoculation medium amount %, cultured at 30°C for 18 h.

[0040] (2) Preparation of MFC inoculum solution: centrifuge the bacterial solution in (1) at 6000 r / min at 4°C for 2 min, remove the supernatant under anaerobic conditions, and obtain bacterial cells, use PBS buffer solution, the composition is ( / L): Na 2 HPO 4 12H 2 O 22.2 g; NaH 2 PO 4 2H 2 O5.93 g; KCl 0.13 g, washed three times, and resuspended in 0.5 mL to obtain the MFC inoculum.

[0041] (3) Preparation of MFC electrode solution: MFC catholyte is PBS buf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Surface areaaaaaaaaaaa
Surface areaaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of microbial fuel cells and relates to a method for evaluating influence on energy metabolism of a living body caused by electromagnetic radiation by using MFC. Thebasis is as follows: an electron transfer chain is an important component of biological cell energy metabolism; a microbial fuel cell can be used for recording current on an electroactive microbial electron transfer chain; electromagnetic field intensity and amplitude of fluctuation of the current generated by the microbial fuel cell are of a linear corresponding relationship. The method comprisesthe following steps: constructing the microbial fuel cell; acquiring current intensity of the same microbial fuel cell under the action of electromagnetic fields of different strengths; constructingan electromagnetic field intensity-current wave amplitude standard curve in a manner of representing the amplitude of fluctuation of the current by standard deviation of current of the same treatmentgroup, wherein the standard curve is used for indicating the influence on the energy metabolism of the living body caused by electromagnetic radiation. According to the method, the influence on healthof the living body caused by electromagnetic radiation is achieved, particularly quantified analysis in the aspect of energy metabolism, and the evaluation result is accurate and reliable.

Description

technical field [0001] The invention belongs to the field of microbial fuel cells, and in particular relates to a method for evaluating the influence of electromagnetic radiation on the energy metabolism of organisms by using MFC. Background technique [0002] The process of electromagnetic field propagating in space at a certain speed is electromagnetic radiation. Electromagnetic radiation is an objective existence. As a kind of energy flow, human beings cannot directly feel it, but it is present all the time and everywhere. When the intensity of electromagnetic radiation exceeds the standard of environmental protection, it will produce negative effects and cause different lesions and hazards of the human body. This part of the radiation exceeding the standard electromagnetic field intensity is called electromagnetic radiation pollution. Different from chemical pollution and biological pollution, electromagnetic radiation pollution is energy pollution, and there will be no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/02C12R1/01
CPCC12Q1/02G01N2333/195
Inventor 余真石振华
Owner FUJIAN ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com