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Isaria javanica for efficiently controlling scale insects

A technology for coryneform spores and scale insects, which is applied in the field of pest biological control and achieves the effects of large spore production, easy preparation and environmental friendliness

Active Publication Date: 2019-03-01
广州市林业和园林科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of the scale insect control technologies such as the existing Egyptian phagocytosis and Pyococcus hibiscus, and to provide a kind of important Egyptian phagocytosis entomopathogenic fungus, i.e. I. javanica ( Isariajavanica), which has high pathogenicity to P. aegypti, can be used as a living biopesticide with high pathogenicity to Homopteran insects such as P. aegypti and Pythococcus hibiscus, and has high efficiency, environmental friendliness, It has the advantages of not easy to produce drug resistance, etc., and has a good application prospect in the prevention and control of the Egyptian scorpion

Method used

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  • Isaria javanica for efficiently controlling scale insects
  • Isaria javanica for efficiently controlling scale insects
  • Isaria javanica for efficiently controlling scale insects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation of I. javanica strain IJID003

[0038] The highly pathogenic fungus was isolated from naturally susceptible Coccoccus aegypti from Bailan in Yuexiu Park, Guangzhou City, Guangdong Province. The method is as follows:

[0039] (1) Isolation of fungi

[0040] The hyphae were picked from the susceptible Coccoccus aegyptis, inoculated on PDA medium for cultivation, and cultivated at 27±1°C for 7 days. The hyphae were picked from the center of the formed colony, purified and cultured on PDA medium, and the purified culture was repeated 3 times, and placed in a 4°C refrigerator for use.

[0041] (2) Fungal inoculation experiment

[0042] Cultivate the isolated fungus on a PDA plate for 7 to 15 days. After the formation of colonies, add 0.02% Tween-80 sterile water to wash the spores, stir on a magnetic stirrer for 15 minutes, and adjust the spore concentration to 1 with a hemocytometer. ×10 7 spores / mL to prepare the spore suspension, use a small manual...

Embodiment 2

[0045] Example 2 Screening of I. javanica strain IJID003

[0046] Entomopathogenic fungi are diverse in terms of genetics, ecology, and biology, and different strains of fungi show significant differences in the pathogenicity of target pests. The screening and acquisition of high-yield and high-quality strains is the first prerequisite for better control effects. The selection of excellent strains mainly considers three indicators, namely sporulation, colony growth rate, and pathogenicity. The present invention uses these three indexes as the main basis to screen excellent highly pathogenic bacterial strains.

[0047] (1) Material preparation

[0048] Eight isolates of purified IJID001, IJID002, IJID003, IJID004, IJID005, IJID006, IJID007, and IJID008 were cultured on PDA plates in a constant temperature incubator at 27±1°C.

[0049] (2) Tested insects and host plants

[0050] Coccus edulis in Egypt came from the subcultured population on the white orchid in the greenhouse...

Embodiment 3

[0065] Identification of embodiment 3 bacterial strain IJID003

[0066] (1) Morphological characteristics of strain IJID003

[0067] The strain IJID003 was inoculated into the center of the PDA medium plate, placed in a constant temperature culture at 27±1°C, and the growth of the colony was observed every day and the color and shape of the colony were recorded. Inoculate the strain IJID003 into the center of another PDA medium plate, insert the sterilized coverslip (1cm×1cm) obliquely into the medium about 1cm away from the inoculation point, and culture at a constant temperature of 27±1°C for 5 days Wait until the hyphae extend to the cover glass, take out the cover glass and place it under an optical microscope to observe the morphology of the hyphae and spores.

[0068] Morphological characteristics of strain IJID003 ( figure 1 and figure 2 ): On the PDA plate, the colony is round, with a diameter between 49mm and 55mm, with concentric ring patterns, and forms grooves ...

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Abstract

The invention discloses isaria javanica for efficiently controlling scale insects. The first study of the isaria javanica for efficiently controlling the scale insects found that the isaria javanica has a very good disease-causing and controlling effect on the scale insects such as icerya aegyptiaca and solenopsis mealybug, an isaria javanica bacterial strain IJID003 with high pathogenicity is screened, the isaria javanica can be used as a living biological pesticide with high pathogenicity on the scale insects, and the isaria javanica for efficiently controlling the scale insects has good broad application prospects in the biological control over the scale insects. The isaria javanica is an entomopathogenic fungus, and a conidia suspension of the isaria javanica can directly act on a wormbody. Compared with current chemical control agents, the isaria javanica has the advantages of efficiency, green, environment-friendly property, environmental protection, high effectiveness and beingnot prone to producing drug resistance, has no pollution to the environment and no residue, and has good market application prospects when the isaria javanica is developed as a biocontrol agent on the biological control over the scale insects.

Description

technical field [0001] The invention belongs to the technical field of pest biological control. More specifically, it relates to a strain of I. javanica that can efficiently control scale insects and Homoptera insects. Background technique [0002] Homoptera is one of the common forestry pests, such as scale insects such as Icerya aegyptiaca (Douglas) and Phenacoccus solenopsis Tinsley. Icerya aegyptiaca (Douglas) is an important pest harmful to forestry and garden plants. It is mainly distributed in Guangdong, Fujian, Zhejiang and Taiwan in my country. Garden plants. As early as the 1980s, this insect was listed as one of the dangerous forestry and garden pests in Guangzhou. Coccus aegypti mainly uses female adults and nymphs to harm the leaf buds, twigs and branches of plants. They gather in groups on the back of leaves or on twigs to suck plant juice, ranging from a few heads to hundreds of heads. At the same time, the excreted honey The soot pollution induced by dew a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 邓嘉茹黄华枝毕可可孙龙华张劲蔼唐立鸿吴超候亚会霍立志阮琳
Owner 广州市林业和园林科学研究院
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