Improved medium for mycoplasma synoviae culture

Abstract

The invention discloses an improved medium for mycoplasma synoviae culture. The improved medium is prepared from the following components: a beef heart infusion medium, phenol red, yeast extract, antibiotics, glucose, essential amino acids, animal serum, beta-nicotinamide adenine dinucleotide and cysteine. The invention further discloses a method for culturing and separating mycoplasma synoviae bythe improved medium. The medium can effectively improve the separation effect of mycoplasma synoviae, density of the mycoplasma in the medium is improved, the influence of other strains on test results is inhibited, the effect of specifically separating the mycoplasma synoviae can be achieved, and results are reliable; detection sensitivity of the mycoplasma synoviae can be improved, culture timecan be shortened, survival time of the mycoplasma synoviae is prolonged, and the medium can be used as a preservation medium; the medium is low in cost and suitable for large-scale production and use.

Description

technical field [0001] The invention relates to the culture technology of Mycoplasma Synoviae in the field of biotechnology, in particular, it is a culture medium suitable for the isolation and cultivation of Mycoplasma Synoviae, so as to study the biological characteristics of Mycoplasma Synoviae. Background technique [0002] Mycoplasma synoviae (Mycoplasma synoviae) is a Gram-negative bacterium, which is pleomorphic spherical or dry, not resistant to heat, and can survive for a long time in a low temperature environment. The main biochemical characteristics are that it can ferment glucose and maltose, produce acid without producing gas, do not ferment lactose, salicin, do not hydrolyze arginine, and do not use urea. Mycoplasma synovialum has a poor ability to survive in the unexpected landscape of the host, and is very sensitive to cold, heat, acid, dryness and conventional disinfectants, and many disinfectants can kill it. Mycoplasma synovialum can cause respiratory dis...

AI Technical Summary

Problems solved by technology

Therefore, Mycoplasma synovialum has caused a huge threat to the poultry breeding industry. Once infected, it will cause poor growth and development of chickens, reduce the quality of broiler chicken carcasses, and reduce the egg production rate, fertilization rate and hatchability of breeder chickens, which will cause huge economic loss

[0003] The isolation and culture of conventional mycoplasma mainly use Frey's medium, but the existing medium conditions cannot support the growth of mycoplasma synovialum, let alone further study the physiology and pathology of mycoplasma synovialum