Compound biological insecticide of veratine and chlorobenzamide
A technology of chlorantraniliprole and biological insecticides, applied in the field of compound insecticides, can solve the problems of low dosage, narrow insecticidal spectrum, insect paralysis, etc., and achieve the activity and synergy of expanding insecticidal spectrum The activity of the insecticidal spectrum and the effect of reducing the cost of use
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experiment example 1
[0018] Experimental example 1: Screening of the compound ratio of veratrine and chlorantraniliprole against melon aphid and determination of co-toxicity coefficient
[0019] 1.1 Test pests and test methods
[0020] The melon aphids raised the melon aphid adults on the cucumber in the greenhouse, and inoculated them on the cucumber seedlings that had grown to 2-3 expanded leaves without insects. After the nymphs were produced, the adults were removed in time, and the cucumber seedlings were placed in an incubator with an ambient temperature of 25°C. Wait until it develops to the 2nd instar nymph as a stand-by.
[0021] 1.2 Drugs to be tested
[0022] 0.5% veratrine soluble (Chengdu New Chaoyang Crop Science Co., Ltd.)
[0023] 5% Chlorantraniliprole SC (Puzun) (produced by DuPont, USA)
[0024] The mixture of veratrine and chlorantraniliprole is prepared from a single dose according to the ratio requirements
[0025] 1.3 Experimental method
[0026] 1.3.1 Toxicity determi...
Embodiment 2
[0048] Example 2: Veratrine and chlorantraniliprole and (1:2) mixture toxicity and co-toxicity coefficient determination to Plutella xylostella 2.1 Test pests and test methods
[0049] Plutella xylostella is reared indoors with germinated seedlings of rapeseed, and is used as a stand-by when it develops to the 2nd instar nymph.
[0050] 2.2 Drugs to be tested
[0051] Same as 1.2
[0052] 2.3 Experimental method
[0053] The concentration of chlorantraniliprole was 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L; the concentration of veratrine was 25mg / L, 50mg / L, 100mg / L, 200 / L, 400mg / L; (1:2) mixture was prepared according to the concentration of 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L. Toxicity was determined by leaf dipping method. Soak the cabbage leaves that have not been exposed to any chemicals for 10 seconds in the prepared 5 concentrations of the drug solution, take them out and dry them, then put them into a petri dish, and insert 10 healthy 2nd instar larvae of the same si...
Embodiment 3
[0059] Example 3: Toxicity of veratrine and chlorantraniliprole and (1:2) mixture to Spodoptera litura and co-toxicity coefficient determination
[0060] 3.1 Test pests and test methods
[0061] Spodoptera litura was reared indoors with artificial feed, and it was used as a stand-by when it developed to the second instar nymph.
[0062] 3.2 Drugs to be tested
[0063] Same as 1.2
[0064] 3.3 Experimental method
[0065] The concentration of chlorantraniliprole was 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L; the concentration of veratrine was 25mg / L, 50mg / L, 100mg / L, 200 / L, 400mg / L; (1:2) mixture was prepared according to the concentration of 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L. Toxicity was determined by leaf dipping method. Soak the cabbage leaves that have not been exposed to any chemicals for 10 seconds in the prepared 5 concentrations of the drug solution, take them out and dry them, then put them into a petri dish, and insert 10 healthy 2nd instar larvae of the same s...
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