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Method for preparing retinal binding protein quality control product

A technology for combining protein and retinol, applied in the field of retinol-binding protein control products, can solve problems such as unfavorable detection errors, and achieve the effects of preventing turbidity, stable performance and good stability

Inactive Publication Date: 2019-02-19
郑州标源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many quality control products are not even made from human serum, but from animal serum. In actual testing, it is inevitable that there will be differences in performance from fresh patient serum, which is not conducive to the real detection error.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of 100ml Retinol Binding Protein Low Level Quality Control

[0019] Its components include: normal human serum 100mL, protein protectant aminopyrine 200mg, preservative Broclin 100mg, retinol binding protein 0.50mg; the effect is equivalent when the protein protectant is polyethylene glycol, gelatin or K30; the preservative uses The effect is equivalent when NaN3, BIT, MIT, BRO or their mixture.

[0020] The preparation method is as follows: collect the remaining fresh serum after the biochemical test and mix it as a matrix solution. It is required to have a clear and transparent appearance, no hemolysis, no jaundice, and HBsAg negative. After detecting the background value, add retinol-binding protein, aminopyrine, and Broclin was then aliquoted and freeze-dried.

Embodiment 2

[0021] Example 2 Preparation of 100ml retinol-binding protein mid-level quality control product

[0022] Its components include: normal human serum 100ml, protein protectant aminopyrine 200mg, preservative Broclin 100mg, retinol binding protein 2.40mg;

[0023] Preparation method is: same as embodiment 1.

Embodiment 3

[0024] Example 3 Preparation of 100ml retinol-binding protein high-level quality control product

[0025] Its components include: normal human serum normal human serum 100ml, protein protectant aminopyrine 200mg, preservative Broclin 100mg, retinol binding protein 4.32mg;

[0026] Preparation method is: same as embodiment 1.

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Abstract

The invention discloses a performance-stable retinal binding protein quality control product, comprising a matrix fluid and a retinol binding protein. The quality control product also comprises a protein protectant and a preservative, wherein the protein protectant is aminopyrine, polyethylene glycol, gelatin or K30, the preservative is one of NaN3, Bronidox, BIT, MIT, BRO or a mixture of two or more substances. The invention has the advantages of stable performance, strong adaptability, and can be applied to mainstream instruments produced by various manufacturers. The quality control productadopts normal human serum or plasma as a matrix liquid, which is consistent with the clinical sample, thereby minimizing the matrix effect and improving the good stability. The added protectant improves the stability of the quality control product, and the addition of the preservative is effective to prevent the turbidity of the quality control product during use.

Description

technical field [0001] The invention relates to a retinol-binding protein control product, in particular to a retinol-binding protein control product with stable performance. Background technique [0002] Retinol-binding protein (RBP) is synthesized by the liver and widely distributed in blood, urine, cerebrospinal fluid and other body fluids. After combining with retinol, it is called retinol (vitamin A) transporter. Human plasma RBP is a single-chain protein with a molecular weight of about 22,000 and 182 amino acid residues. The production of RBP in healthy people is relatively constant, and the synthesis rate of adults is about 5mg / kg body weight per day. Most of the RBP in the plasma is combined with prealbumin to form a complex, which cannot be filtered by the glomerulus. When RBP supplies retinol to the target organ, it becomes deretinol RBP (Apo-RBP), and the binding affinity with prealbumin decreases and dissociates, which is called free RBP, accounting for about ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李双玲赵晓转王新明刘功成
Owner 郑州标源生物科技有限公司
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