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Liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice

A technology of isotope mass spectrometry and liquid chromatography, which is applied in the field of liquid chromatography-isotope mass spectrometry for the authenticity identification of grape juice, can solve the problems of high consumption of reagents and complicated processes, and achieves high sensitivity, high accuracy and feasibility of the instrument. strong effect

Inactive Publication Date: 2019-02-19
JIANGSU SINOGRAPHY TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latest report uses elemental analysis-carbon isotope ratio mass spectrometry to analyze the δ of sugars and organic acids 13 C value to identify true and false grape juice, but the sample processing involves the precipitation of organic acids, the process is complicated, and the reagent consumption is high

Method used

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  • Liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice
  • Liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice
  • Liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1. Preparation of pure grape juice: The raw materials of real grape juice come from enterprises or large supermarkets. A total of 50 kinds of fresh, plump, no disease spots, and whole grapes are selected, and the skins and seeds are kept, and put into a juicer to extract pure grape juice. , aliquoted and stored frozen at -18°C for later use.

[0026] S2. Grape juice sample processing: 30 commercially available grape juice samples (pure or with added sugar) for identification and analysis were purchased from different supermarkets.

[0027] S3. For all the grape juices, weigh 0.3 mg respectively, dilute to 20 mL with ultrapure water, vortex fully, filter through a 0.22 μm water phase filter membrane, and perform liquid phase-isotope ratio mass spectrometry analysis.

[0028] S4. Experimental instruments: Survey liquid chromatograph, DELTA V Advantage isotope mass spectrometer: (Thermo Scientific, USA)

[0029] S5, liquid chromatography (HPLC) conditions: chromatographi...

Embodiment 2

[0037] S1. Preparation of pure grape juice: The raw materials of real grape juice come from enterprises or large supermarkets. A total of 50 kinds of fresh, plump, no disease spots, and whole grapes are selected, and the skins and seeds are kept, and put into a juicer to extract pure grape juice. , aliquoted and stored frozen at -18°C for later use.

[0038] S2. Grape juice sample processing: 30 commercially available grape juice samples (pure or with added sugar) for identification and analysis were purchased from different supermarkets.

[0039] S3. For all the grape juices, weigh 0.35 mg respectively, dilute to 20 mL with ultrapure water, vortex fully, filter through a 0.22 μm water phase filter membrane, and perform liquid phase-isotope ratio mass spectrometry analysis.

[0040] S4. Experimental instruments: Survey liquid chromatograph, DELTA V Advantage isotope mass spectrometer: (Thermo Scientific, USA)

[0041] S5, liquid chromatography (HPLC) conditions: chromatograph...

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Abstract

The invention provides a liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice, in particular to the technical field of detection. The invention comprises the steps of: establishing a corresponding isotope difference database by determining the Delta<13>C value of fructose, glucose, disaccharide and polysaccharide (if contained) in pure grape juice;and judging the authenticity of the grape juice to be tested by comparing the isotope difference value between the grape juice to be tested and the pure grape juice. According to the liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice, high performance liquid chromatograph and Delta V Advantage stable isotope ratio mass spectrometer (HPLC-IRMS) isadopted; the instrument has high sensitivity and strong feasibility, so it is only necessary to measure the Delta<13>C of the sugar in the grape juice online; the amount of the grape juice sample usedis small, so the sample processing is simple and quick; the source of the syrup for adulteration does not need to be clear, and the accuracy of the qualitative identification result is high.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a liquid chromatography-isotope mass spectrometry method for authenticity identification of grape juice. Background technique [0002] Grapes and their products contain a variety of bioactive components (sugars, organic acids, polyphenols, amino acids, etc.), which have anti-oxidation and anti-aging effects, and are effective in reducing the occurrence of arteriosclerosis, cardiovascular diseases, Parkinson's disease, cancer, cataracts, etc. The rate plays an important role, so it is very popular among consumers. There are many brands and varieties of grape juice sold in the market, and the prices are uneven. Many manufacturers often add cheap sugar or syrup in order to make high profits, but they still advertise that they use pure grape juice raw materials, which damages the rights and health of consumers. It is very necessary to invent a simple and efficient dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐锦忠鲁惠玲王平石星宇
Owner JIANGSU SINOGRAPHY TESTING
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