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Nanostraw well insert devices for improved cell transfection and viability

A technology for inserting devices and nanotubes, applied in nanotechnology for materials and surface science, nanotechnology, animal cells, etc., can solve the problems of lack of general tools in the field of determination and transfection

Active Publication Date: 2019-02-05
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, despite nearly half a century of technological innovation, development, and optimization, the field of transfection lacks a universal tool for delivery into cells; that is, existing methods all have advantages and disadvantages that are highly dependent on Experimental Design and Objectives

Method used

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  • Nanostraw well insert devices for improved cell transfection and viability
  • Nanostraw well insert devices for improved cell transfection and viability
  • Nanostraw well insert devices for improved cell transfection and viability

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Embodiment Construction

[0044] Transfection, or the transport and integration of external material (usually nucleic acids) into the cytosol and / or nucleus of living cells, is a fundamental technique widely used in modern biological, biomedical, and biotechnological approaches, including cellular reprogramming, Intracellular imaging and sensing, molecular agriculture, siRNA knockdown, drug screening and drug therapy.

[0045]VanDersarl et al. have previously reported a simple biomimetic nanostructure or "nanotube" that can establish a continuous fluid pathway into the cell interior for intracellular delivery and extraction of molecular cargo. Nanotubes are metal oxide nanotube structures with diameters on the order of 100 nm embedded in widely used polymer membrane filters. Cells cultured on nanotube devices are spontaneously permeable, providing a stable external handle for delivering fluid material substances into cells. Nanotubes have been demonstrated to successfully transport molecules from ions...

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Abstract

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane froma fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to US Provisional Patent Application No. 62 / 347,928, filed June 9, 2016, which is hereby incorporated by reference in its entirety. [0003] incorporated by reference [0004] All publications and patent applications mentioned in this specification are herein incorporated by reference in their entirety to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. [0005] Statement Regarding Federally Funded Research [0006] This invention was made with government support under contract 1549696 awarded by the National Science Foundation. The government has certain rights in this invention. Background technique [0007] Transfection, or generally the delivery of molecular species across a lipid bilayer and into the cytosol or nucleus, is a powerful analytical tool with numerous applica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10A61K9/127C12N5/07C25D11/34
CPCC25D11/34B82Y5/00B82Y30/00B82Y40/00C23C16/403C23C16/45525C23C16/45555C23C16/56A61K9/10A61K9/127C12M23/12C12M35/02C12N15/87B01L3/508B01L2300/0609B01L2300/0672B01L2300/0832B01L2300/12B01L2400/0415C12M25/02C12M25/10C12M29/16C12N5/0075C12N2531/00C12N2533/00C12N2539/00C23C16/402C23C16/405C23C16/45553
Inventor R·T·斯沃博达Y·曹S·李尔-欧迪兹S·罗斯克特N·A·米洛施
Owner THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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