Method for isolating edible fungus strains

Abstract

The invention relates to a method for isolating edible fungus strains. The method comprises the following steps: performing exposure of a fresh aseptic tissue of a fruiting body, cutting off a block from the surface of the aseptic tissue with a sharp tip, inserting the shape tip obliquely into the tissue, performing isolation with the shape tip, allowing a slope in a test tube to be downward, pulling out a tampon, clamping the tissue block with tweezers, burning the tissue block over flame lightly and to and fro, putting the burned tissue block into the test tube, covering the test tube with the tampon, tilting the test tube finally, allowing an isolation culture medium to face downwards, shaking the tissue block gently so as to make the tissue block slide directly from the mouth of the test tube to the bottom of the test tube containing the culture medium and be stuck between the culture medium and the test tube, and then putting the test tube at a temperature of 22-28 DEG C, whereingermination of hyphae occurs in 3 days, and transfer of the strains can be conducted in 8-12 days. According to the isolating method, operation can be conducted in an open environment without specialequipment, and in addition, alcohol and other disinfectants are not needed for the surface treatment, and direct clamping with the tweezers and even burning over the flame of an alcohol lamp are conducted, so that the surface dust and hybrid bacteria are burned by the flame; the tissue block does not pass over the culture medium, so that the contamination rate is reduced greatly.

Description

technical field [0001] The invention relates to a method for germination and separation of edible fungus strains by inoculating block blocks with culture medium upside down, which belongs to the separation method of microbial strains. Background technique [0002] Strain isolation is the operation method of transferring the local tissues, spores or basal hyphae of valuable fruiting bodies to the slant test tube culture medium to obtain pure cultured mycelia. There are two ways to obtain fungal resources: field collection and cultivation site collection, among which the collection of field specimens is mainly applicable to the domestication of wild species of edible fungi, genetic breeding, physiological ecology, and the isolation of various first-class species for research. And in the separation of the first-level species in the cultivation of edible fungi, the source of its seed mushrooms is mainly obtained from the cultivation field through seed retention. [0003] Edible...

AI Technical Summary

Problems solved by technology

[0005] This method and the derived method all adhere the separated tissue pieces to the center of the test tube culture medium, and the tissue pieces are easy to move during the moving process, which increases the chance of contamination

In addition, during the separation process, the tissue block is operated and fixed above the culture medium, which also increases the chance of inoculation tool not fully sterilized miscellaneous bacteria colonizing on the surface of the culture medium

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