Edible fungus culture separating method

Abstract

The invention relates to an edible fungus culture separating method. The method includes the steps: enabling a fresh aseptic tissue of a sporocarp to expose, blocking the aseptic tissue surface with a sharp pointed end, and obliquely inserting the sharp pointed end into the tissue prior to breaking off the blocked aseptic tissue surface with the pointed end; setting a test tube slant downward, pulling up a cotton plug, using tweezers to clamp a tissue block to enable the same to be lightly singed for 2-3 times above a flame back and forth, and plugging the cotton plug; and finally, inclining a test tube, setting an isolation medium downward, slightly shaking the tissue block to enable the same to directly slip from a test tube opening to the bottom of the culture medium test tube to be clamped between a culture medium and the test tube, then placing the test tube for 3 days at the temperature of 22-28 DEG C so that hyphae can be germinated, and culture rotation can be performed after 8-12 days. The edible fungus culture separating method can be operated in an open environment without special equipment; surface treatment needs no disinfectant (for example: alcohol), the tissue block is directly clamped by the tweezers to uniformly pass through the flame of an alcohol burner, so that dust and other bacteria on the surface can be lightly singed by the flame; and the tissue block does not need to pass through a position above the culture medium so that contamination ratio is greatly decreased.

Description

technical field [0001] The invention relates to a method for germination and separation of edible fungus strains by inoculating block blocks with culture medium upside down, which belongs to the separation method of microbial strains. Background technique [0002] Strain isolation is the operation method of transferring the local tissues, spores or basal hyphae of valuable fruiting bodies to the slant test tube culture medium to obtain pure cultured mycelia. There are two ways to obtain fungal resources: field collection and cultivation site collection, among which the collection of field specimens is mainly applicable to the domestication of wild species of edible fungi, genetic breeding, physiological ecology, and the isolation of various first-class species for research. And in the separation of the first-level species in the cultivation of edible fungi, the source of its seed mushrooms is mainly obtained from the cultivation field through seed retention. [0003] Edible...

AI Technical Summary

Problems solved by technology

[0005] This method and the derived method all adhere the separated tissue pieces to the center of the test tube culture medium, and the tissue pieces are easy to move during the moving process, which increases the chance of contamination

In addition, during the separation process, the tissue block is operated and fixed above the culture medium, which also increases the chance of inoculation tool not fully sterilized miscellaneous bacteria colonizing on the surface of the culture medium

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