Dynamic cell culture apparatus and cell culture system

Abstract

A dynamic cell culture apparatus and a cell culture system are provided, the dynamic cell incubator includes a cuboidal shell, a plurality of layers of culture chambers distributed from the bottom tothe top and parallel to each other are arranged inside the shell, the liquid inlet chamber and the liquid outlet chamber are respectively arranged at two opposite sides of the shell, the two ends of the culture chamber of any layer are respectively communicated with the liquid inlet chamber and the liquid outlet chamber, the other two opposite sides of the housing are respectively provided with aliquid inlet (11) and a liquid outlet (31), wherein the line connecting the liquid inlet (11) and the liquid outlet (31) is a body diagonal line of the housing, the liquid inlet (11) is communicated with the liquid inlet chamber, and the liquid outlet (31) is communicated with the liquid outlet chamber. The dynamic cell culture apparatus provided by the invention has high use convenience, is suitable for manual operation, can reduce the pollution risk in the cell culture process, and can realize a large number of cells to be cultured and harvested at one time.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a dynamic cell culture device and a cell culture system. Background technique [0002] In the past ten years, due to the rapid development of the biological products industry, the traditional method of obtaining biological products from animal tissues through biochemical technology has been far from meeting the needs of the market. Large-scale culture of animal cells in vitro to express specific proteins, Monoclonal antibody, interferon and virus vaccine products have become the most common technology at present. [0003] Currently commonly used cell culture devices include conventional cell culture dishes, plates, bottle cultures, spinner bottle cultures, large bioreactors, etc. The above cell culture devices have the following defects: [0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested i...

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Problems solved by technology

[0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested in a single culture is small. To obtain a large number of cells requires a large increase in the number of devices and repeated cultures, and repeated cultures require a lot of materials and labor. and time;

[0005] 2) During the cultivation process, the preparation of culture medium, addition of liquid, and harvesting of cells and cultures are all manual operations step by step. There is a lack of a fully enclosed, automated, programmed, continuous and efficient integrated culture system, which greatly reduces the risk of contamination during the culture process. improve;

[0006] 3) The cell factories currently on the market can achieve the purpose of harvesting a large number of cells in one culture, but they take up a lot of space, and the cultivation process requires a large amount of culture medium, trypsin and other reagents. Taking the ten-layer cell factory as an example, the product height reaches 20cm. After filling the culture solution, the weight of the product increases significantly, and manual operation is very difficult. The whole process needs to be assisted by manipulators. The product is large in size and does not fit the laboratory incubator. It is necessary to purchase a special incubator, which will greatly increase equipment investment and consume a lot. Manpower, time, and space limit its widespread use

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