Kit and method for identifying pollination donors for citrus pomelo based on s genotype

A kit and genotyping technology, applied in the field of citrus pomelo planting, can solve the problems of reporting and immaturity, and achieve the effects of accurate results, simple operation, and fast identification methods

Active Publication Date: 2020-08-07
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional aniline blue dyeing method is mostly used on crops with small styles such as Solanaceae and Rosaceae. The sampling time after pollination, the formula of tissue fixative, and the time of tissue softening and dyeing are all easy to master. However, the style of pomelo is relatively large. No mature aniline blue staining method has been reported for large styles

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  • Kit and method for identifying pollination donors for citrus pomelo based on s genotype
  • Kit and method for identifying pollination donors for citrus pomelo based on s genotype
  • Kit and method for identifying pollination donors for citrus pomelo based on s genotype

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Embodiment Construction

[0027] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0028] 1. Identification of S gene

[0029] The inventor conducted research on a large number of citrus pomelo plant materials and identified 9 different S genes. And the primers specially designed for these 9 kinds of S genes are shown in Table 1.

[0030] Table 1 Primer pairs 1-9 and related gene information

[0031]

[0032]

[0033] 2. Identification of S genotypes for 14 pomelo plants

[0034] 14 pomelo varieties were selected as the materials to be tested, as shown in Table 2.

[0035] Table 2 Summary of information on 14 pomelo varieties

[0036]

[0037] Genomic DNA of the samples to be tested was extracted using the CTAB method. Using these genomes as templates, PCR amplification was performed...

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Abstract

The invention relates to a kit for authenticating a pollination donor for citrus grandis based on S-genotype. The kit for authenticating the pollination donor for the citrus grandis based on the S-genotype comprises an S gene detection reagent group. The invention further relates to a method for authenticating the pollination donor for the citrus grandis based on the S-genotype. The method for authenticating the pollination donor for the citrus grandis based on the S-genotype is characterized in that the kit is used for detecting S genes of target citrus grandis plants and a candidate pollination donor, when the S gene of target citrus grandis plants is different from the S gene of the candidate pollination donor, it is judged that the candidate pollination donor can be used as the pollination donor of the target citrus grandis plants, when the S gene of target citrus grandis plants is the same as the S gene of the candidate pollination donor, it is judged that the e candidate pollination donor cannot be used as the pollination donor of the target citrus grandis plants. The authenticating method is fast and convenient, low in price, accurate and provides a staining method suitablefor large-scale styles, and facilitates observation of growing status of pollen tubes after pollination.

Description

technical field [0001] The invention relates to the technical field of planting citrus pomelo plants, and more particularly relates to a kit and identification method for identifying pollination donors for target citrus pomelo plants based on the S genotype. Background technique [0002] Citrus is the largest category of fruit in the world, and its industrial economic value is huge. Citrus grandis is one of the important cultivars, with Shatian pomelo, Guanxi honey pomelo and other Chinese traditional fine pomelo varieties as the main varieties, with a relatively large planting area. In the actual production process, it is found that most pomelo varieties have a self-incompatibility mechanism, which seriously affects the fruit yield, so the selection of pollination trees is very important in pomelo production and cultivation. Studies have shown that the self-incompatibility traits of pomelo plants are genetically controlled by multiple alleles at a single locus, namely the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895G01N1/30
CPCC12Q1/6895C12Q2600/156G01N1/30
Inventor 柴利军梁梅曹宗洪邓秀新
Owner HUAZHONG AGRI UNIV
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