Sweet potato leaf antioxidant active substance
A technology of antioxidant activity and sweet potato leaves, which is applied in the direction of anti-toxic agents, drug combinations, plant raw materials, etc., can solve the problems of reduced antioxidant activity of the final product, complicated and complicated preparation methods, and waste, so as to improve the total antioxidant capacity and improve Hydrophilicity, effect of reducing polarity
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Embodiment 1
[0019] A kind of sweet potato leaf antioxidant active substance, sweet potato leaf polysaccharide content in the sweet potato leaf antioxidant active substance is 64.5%, polyphenols content is 12.8%, flavonoids content is 8.4%, sweet potato leaf antioxidant active substance is to DPPH free radical, ·OH and ·O 2- It has a strong scavenging effect, which can effectively improve the total antioxidant capacity, antioxidant enzyme activity and GSH content of rats, thereby reducing the peroxidation of free radicals on cell membrane lipids, reducing the content of MDA in the body, and preventing spontaneous lipid overload in the liver. Oxidation shows an inhibitory effect, so the antioxidant active substances of sweet potato leaves can be used as an antioxidant active ingredient to remove excess free radicals in the body.
Embodiment 2
[0021] A kind of sweet potato leaf antioxidant active substance, its preparation method comprises the following steps:
[0022]1) Select healthy, non-rotten and non-yellowing sweet potato leaves, wash them, dry them, pass through a 100-mesh sieve after crushing, add methanol and reflux at 75°C for 3 hours to degrease, take the filter residue after suction filtration, and evaporate the methanol naturally; The liquid ratio is 1:45 and mixed with ultrapure water, and the extraction time is 45min under the conditions of microwave power of 350W and temperature of 65°C, and the filtrate is obtained to obtain the sweet potato leaf solution; 2) Take the sweet potato leaf solution, set the ultrasonic frequency to 20KHz, power Extraction conditions of 400W, temperature 55°C, extraction for 35min each time, leaching three times, then combining, evaporating, and concentrating the filtrate; after cooling, dilute with absolute ethanol and let it stand for 24h, filter out the precipitate, and...
Embodiment 3
[0024] A kind of sweet potato leaf antioxidant active substance, its preparation method comprises the following steps:
[0025] 1) Degreasing: Select healthy, non-rotten and non-yellowing sweet potato leaves, clean them, dry them, crush them through a 140-mesh sieve, add methanol and reflux at 78°C for 5 hours to degrease, take the filter residue after suction filtration, and evaporate methanol to dryness naturally; According to the ratio of solid to liquid 1:50, add ultrapure water to mix, extract for 60 minutes under the conditions of microwave power of 400W and temperature of 70°C, take the filtrate to obtain sweet potato leaf solution; degreasing with methanol not only greatly improves the antioxidant capacity of sweet potato leaf solution The content of active substances is conducive to its further extraction, and can also prevent the pollution and degradation of lipid substances and antioxidant substances;
[0026] 2) Extraction: take the sweet potato leaf solution, set ...
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