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Sweet potato leaf antioxidant active substance

A technology of antioxidant activity and sweet potato leaves, which is applied in the direction of anti-toxic agents, drug combinations, plant raw materials, etc., can solve the problems of reduced antioxidant activity of the final product, complicated and complicated preparation methods, and waste, so as to improve the total antioxidant capacity and improve Hydrophilicity, effect of reducing polarity

Inactive Publication Date: 2019-01-22
金华市飞凌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, technologies such as vacuum decompression, resin adsorption, and spray drying have been used in the inventive method, and its preparation method is complicated, and the sweet potato polysaccharide, which accounts for the highest component in the antioxidant active ingredients of sweet potato leaves, is lost in vain, which not only causes waste , and the antioxidant activity of the final product was also significantly reduced

Method used

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  • Sweet potato leaf antioxidant active substance
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of sweet potato leaf antioxidant active substance, sweet potato leaf polysaccharide content in the sweet potato leaf antioxidant active substance is 64.5%, polyphenols content is 12.8%, flavonoids content is 8.4%, sweet potato leaf antioxidant active substance is to DPPH free radical, ·OH and ·O 2- It has a strong scavenging effect, which can effectively improve the total antioxidant capacity, antioxidant enzyme activity and GSH content of rats, thereby reducing the peroxidation of free radicals on cell membrane lipids, reducing the content of MDA in the body, and preventing spontaneous lipid overload in the liver. Oxidation shows an inhibitory effect, so the antioxidant active substances of sweet potato leaves can be used as an antioxidant active ingredient to remove excess free radicals in the body.

Embodiment 2

[0021] A kind of sweet potato leaf antioxidant active substance, its preparation method comprises the following steps:

[0022]1) Select healthy, non-rotten and non-yellowing sweet potato leaves, wash them, dry them, pass through a 100-mesh sieve after crushing, add methanol and reflux at 75°C for 3 hours to degrease, take the filter residue after suction filtration, and evaporate the methanol naturally; The liquid ratio is 1:45 and mixed with ultrapure water, and the extraction time is 45min under the conditions of microwave power of 350W and temperature of 65°C, and the filtrate is obtained to obtain the sweet potato leaf solution; 2) Take the sweet potato leaf solution, set the ultrasonic frequency to 20KHz, power Extraction conditions of 400W, temperature 55°C, extraction for 35min each time, leaching three times, then combining, evaporating, and concentrating the filtrate; after cooling, dilute with absolute ethanol and let it stand for 24h, filter out the precipitate, and...

Embodiment 3

[0024] A kind of sweet potato leaf antioxidant active substance, its preparation method comprises the following steps:

[0025] 1) Degreasing: Select healthy, non-rotten and non-yellowing sweet potato leaves, clean them, dry them, crush them through a 140-mesh sieve, add methanol and reflux at 78°C for 5 hours to degrease, take the filter residue after suction filtration, and evaporate methanol to dryness naturally; According to the ratio of solid to liquid 1:50, add ultrapure water to mix, extract for 60 minutes under the conditions of microwave power of 400W and temperature of 70°C, take the filtrate to obtain sweet potato leaf solution; degreasing with methanol not only greatly improves the antioxidant capacity of sweet potato leaf solution The content of active substances is conducive to its further extraction, and can also prevent the pollution and degradation of lipid substances and antioxidant substances;

[0026] 2) Extraction: take the sweet potato leaf solution, set ...

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Abstract

The invention discloses a sweet potato leaf antioxidant active substance. The sweet potato leaf antioxidant active substance contains sweet potato leaf polysaccharide, polyphenols and flavonoids. A preparation method of the sweet potato leaf antioxidant active substance comprises the steps of degreasing, extraction, deproteinization and decoloration, specifically, firstly, sweet potato leaf powderis degreased, then subjected to microwave extraction and ultrasound extraction, then is subjected to deproteinization through an ultra-pure water solution of trichloroethanoic acid and 10-camphorsulfonic acid, and finally subjected to decoloration treatment through medicinal activated carbon powder, so that the sweet potato leaf antioxidant active substance is obtained. The sweet potato leaf antioxidant active substance has the beneficial effects that the sweet potato leaf antioxidant active substance has the high clearing effect on DPPH free radicals, .OH and .O<2->, an extraction technologyof the sweet potato leaf antioxidant active substance is simple and easy to do, in the extraction process, the active substance can be effectively prevented from being degraded and separated and removed mistakenly, and the content of effective utility components of antioxidant active substances is greatly increased.

Description

technical field [0001] The invention relates to the field of deep processing of sweet potato leaves, in particular to an antioxidant active substance of sweet potato leaves. technical background [0002] Sweet potato (scientific name: Dioscorea esculenta (Lour.) Burkill ): Also known as sweet potato, it is a twining herbaceous vine of the genus Dioscorea of ​​Convolvulaceae. The top branch of the underground tuber is swollen into an ovoid tuber with light yellow outer skin and smooth. Stems left-handed, spiny at base, covered with T-shaped pilose. Single leaves alternate, broad heart-shaped; male inflorescences are spikes, solitary, male flowers sessile or with very short stalks; bracts ovate, apex acuminate; perianth shallow cup-shaped, pubescent; capsule three Prismatic, apex slightly concave, base truncate, each edge winged; seeds round, winged. Flowering in early summer. Sweet potato stems and leaves are rich in nutrients and are good feed for livestock. According ...

Claims

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Application Information

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IPC IPC(8): A61K36/39A61P39/06A61K127/00
CPCA61K36/39A61K2236/331A61K2236/39A61K2236/55A61P39/06
Inventor 金韶京
Owner 金华市飞凌生物科技有限公司
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