Method for cultivating caryoplasmic hybrid wheat

A hybrid wheat and plant technology, applied in the field of nucleocytoplasmic hybrid wheat cultivation, can solve the problems of long nuclear replacement cycle and the like

Inactive Publication Date: 2002-10-16
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

Therefore, not only the cycle of nuclear replacement is long, but also it will cause crossover between wheat and wild species chromosomes

Method used

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Examples

Experimental program
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Embodiment Construction

[0021] Aegilops Squarrosa line 011 was used as the female parent. Cultivated under natural conditions. Select 182 florets and pull out the tassels from the leaflets on the plant before the tassels come out naturally, and cut off 1 / 3 of the upper part of the glume of the florets to trim the tassels. After emasculation, pollen of common wheat (Triticum aestivum) variety Kenya (Kenya) was inoculated. Pollination was carried out at 9-10 am, and pollination was bagged and isolated. On the second day, pollination was repeated once with the same male parent pollen. 6 hours after the second pollination, 1.0% p-chlorophenoxyacetic acid solution was dripped on the stigma of the pollinated female parent with a dropper, 3 drops per stigma, and the dropping point was repeated once after 24 hours. Another 180 florets were selected as controls, and treated as above, except that distilled water was used to replace 1.0% p-chlorophenoxyacetic acid solution and dropped on the stigma of the fe...

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Abstract

The present invention provides a method for culturing karyoplasmic hybrid wheat. Its method includes the following steps: adopting young female parent stigma, repeated pollination, using p-chlorophenoxy acetic acid solution to treat the pollinated female parent stigma timely to make the setting percentage of normal seed raised by 25-38 times. Said invention implements somatic chromosome number inspection of BC1 and BC2 plants, and promptly selects 2n=42 plants, and uses them as female parent to make replacement backcross once or twice, so that it can implement nuclear replacement program, andits breeding period can be reduced by 3-4 years.

Description

technical field [0001] The invention relates to a method for cultivating nucleoplasmic hybrid wheat. Background technique [0002] Nuclear-cytoplasmic hybrid breeding is a new breeding technology. Its innovation and characteristics lie in the development from purely paying attention to nuclear genes to paying attention to the interaction effects of cytoplasm and nuclear-plasma at the same time. Wheat nucleoplasmic hybrid breeding can combine the advantages of pure line breeding and cytoplasmic male sterility heterosis breeding, has great potential for increasing yield, and is expected to make a breakthrough in wheat variety improvement. [0003] Breeding wheat nuclear hybrids requires replacing or transplanting the nucleus of wheat into the cytoplasm of another species to breed wheat with heterologous cytoplasm. For higher plants, although some people have tried to use nuclear or organelle transplantation, induced male nucleus to develop alone, chromosome disappearance and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 吴郁文刘春光张炎张翠兰任树新侯宁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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