Method for cultivating caryoplasmic hybrid wheat

Abstract

The present invention provides a method for culturing karyoplasmic hybrid wheat. Its method includes the following steps: adopting young female parent stigma, repeated pollination, using p-chlorophenoxy acetic acid solution to treat the pollinated female parent stigma timely to make the setting percentage of normal seed raised by 25-38 times. Said invention implements somatic chromosome number inspection of BC1 and BC2 plants, and promptly selects 2n=42 plants, and uses them as female parent to make replacement backcross once or twice, so that it can implement nuclear replacement program, andits breeding period can be reduced by 3-4 years.

Description

technical field [0001] The invention relates to a method for cultivating nucleoplasmic hybrid wheat. Background technique [0002] Nuclear-cytoplasmic hybrid breeding is a new breeding technology. Its innovation and characteristics lie in the development from purely paying attention to nuclear genes to paying attention to the interaction effects of cytoplasm and nuclear-plasma at the same time. Wheat nucleoplasmic hybrid breeding can combine the advantages of pure line breeding and cytoplasmic male sterility heterosis breeding, has great potential for increasing yield, and is expected to make a breakthrough in wheat variety improvement. [0003] Breeding wheat nuclear hybrids requires replacing or transplanting the nucleus of wheat into the cytoplasm of another species to breed wheat with heterologous cytoplasm. For higher plants, although some people have tried to use nuclear or organelle transplantation, induced male nucleus to develop alone, chromosome disappearance and ...

AI Technical Summary

Problems solved by technology

Therefore, not only the cycle of nuclear replacement is long, but also it will cause crossover between wheat and wild species chromosomes