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Content detection method for tanshinone IIA in Yixinshu traditional Chinese medicine preparation

A technology of traditional Chinese medicine preparation and tanshinone, which is applied in the field of traditional Chinese medicine and can solve problems such as not being able to fully reflect product quality status and the like

Inactive Publication Date: 2019-01-18
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Due to the large number of active ingredients, sensitive detection methods are needed to detect the active ingredients of Yixinshu traditional Chinese medicine preparations, but the existing detection methods only include traits, 2 color identification and related detection items under the inspection items, and no medicinal materials are tested. Qualitative or quantitative detection cannot fully reflect the quality status of the product. The present invention has established a method for the determination of tanshinone IIA content in the product for the monarch drug Salvia miltiorrhiza, so that more effective quality analysis can be carried out on the product, and the quality status of the product can be more comprehensively reflected. The quality stability of the product is guaranteed, and the method saves testing costs at the same time

Method used

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  • Content detection method for tanshinone IIA in Yixinshu traditional Chinese medicine preparation
  • Content detection method for tanshinone IIA in Yixinshu traditional Chinese medicine preparation
  • Content detection method for tanshinone IIA in Yixinshu traditional Chinese medicine preparation

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Experimental program
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Effect test

Embodiment 1

[0070] Determination of Tanshinone IIA in Yixinshu Capsules (prepared according to Pharmacopoeia method):

[0071] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile: methanol: 0.5% ammonium acetate = 67:12:21 is used as mobile phase; flow rate is 0.8-1.0mL min -1 ;The detection wavelength is 270nm; the column temperature is 25°C; the number of theoretical plates should not be less than 2000 based on the peak of tanshinone IIA;

[0072] (2) Preparation of reference substance solution: accurately weigh an appropriate amount of tanshinone IIA reference substance, add methanol to dissolve, and make 1 mg / mL tanshinone IIA reference substance solution;

[0073] (3) Preparation of the test solution: Accurately weigh 14g of the contents of Yixinshu Capsules, accurately add 100mL of methanol, weigh the weight, ultrasonically extract for 40min, make up the weight with methanol, and filter; take the subsequent fi...

Embodiment 2

[0076] Determination of Tanshinone IIA in Yixinshu Tablets (prepared according to Pharmacopoeia method):

[0077] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use 0.04mol / L sodium dihydrogen phosphate: acetonitrile = 20:80 as mobile phase; flow rate 0.8~1.0mL min -1 ;The detection wavelength is 270nm; the column temperature is 25°C; the number of theoretical plates should not be less than 2000 based on the peak of tanshinone IIA;

[0078] (2) Preparation of reference substance solution: accurately weigh an appropriate amount of tanshinone IIA reference substance, add methanol to dissolve, and make 1 mg / mL tanshinone IIA reference substance solution;

[0079] (3) Preparation of the test solution: Accurately weigh 14g of Yixinshu tablet, accurately add 100mL of methanol, weigh, ultrasonically extract for 60min, make up the weight with methanol, filter; take the subsequent filtrate, which is the test solution ; (4) ...

Embodiment 3

[0081] Determination of Tanshinone IIA in Yixinshu Capsules (prepared according to the method described in Pharmacopoeia 2015 Edition):

[0082] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile: methanol: 0.01% ammonium acetate = 70:15:15 is used as mobile phase; flow rate is 1.5mL min -1 ;The detection wavelength is 270nm; the column temperature is 40°C; the number of theoretical plates should not be less than 2000 based on the peak of tanshinone IIA;

[0083] (2) Preparation of reference substance solution: accurately weigh an appropriate amount of tanshinone IIA reference substance, add methanol to dissolve, and make 1 mg / mL tanshinone IIA reference substance solution;

[0084] (3) Preparation of the test solution: Accurately weigh 14g of the contents of Yixinshu Capsules, accurately add 100mL of methanol, weigh, ultrasonically extract for 30min, make up the weight with methanol, and filter; take t...

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Abstract

The invention relates to a content detection method for tanshinone IIA in Yixinshu traditional Chinese medicine preparation. A high performance liquid chromatography method is adopted for measurement,a tanshinone IIA reference substance is taken as a reference, and the high performance liquid chromatography method is adopted to measure the contents of tanshinone IIA in the Yixinshu traditional Chinese medicine preparation on the wave length of 250-300nm. Compared with the prior art, the method measures the contents of astragaloside in the Yixinshu traditional Chinese medicine preparation through the high performance liquid chromatography method. The measurement method has the advantages of being high in specificity, good in repeatability, high in recovery rate, high in stability and accurate in measurement results, a purpose of effectively controlling medicine quality is achieved, product quality is guaranteed to be stable, and clinical medicine utilization is guaranteed to be safe and effective.

Description

technical field [0001] The invention relates to a method for detecting the content of tanshinone IIA in Yixinshu traditional Chinese medicine preparation, which belongs to the technical field of traditional Chinese medicine. Background technique [0002] Yixinshu traditional Chinese medicine preparation has the functions of nourishing qi and nourishing yin, activating blood and rejuvenating pulse; it is used for deficiency of both qi and yin, internal resistance of heart and blood, chest obstruction and heartache, chest tightness and discomfort, palpitation and pulse knot. It has the characteristics of high drug loading, fast onset, no peculiar smell, and easy to carry. Ginseng, Astragalus, Ophiopogon japonicus, Schisandra chinensis, Salvia miltiorrhiza, and Chuanxiong 6 herbs. Among them, ginseng has the functions of nourishing vitality, nourishing blood, nourishing the heart and calming the nerves. Raw sun-dried ginseng can improve brain, physical activity and immune func...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 安怀略
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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