Pseudomonas mosselii and application thereof
A technology of Pseudomonas morganii and Pseudomonas, which is applied in the field of Pseudomonas, can solve problems such as environmental pollution, pesticide residues, and drug resistance of pathogenic bacteria, and achieve excellent antibacterial effect and good antibacterial effect Effect
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[0044] 1. Isolation and purification of strains
[0045] Five points were randomly selected from the rice planting area of the Fuyang Base of the China National Rice Research Institute to dig out the rice roots. The floating soil on the root system was gently washed with running water. The soil closely attached to the root system was washed off by ultrasonic vibration. Dilute the soil suspension under gradient and apply it to an LB petri dish. Place the petri dish in a constant temperature incubator at 28°C. Whenever a colony grows, pick different morphological colonies on a fresh LB medium plate for streaking purification , And then pick a single colony on the LB solid slope at 4℃ for short-term storage for activity determination.
[0046] The purified single colony was inoculated into LB liquid, and after shaking culture at 28°C overnight, the bacterial liquid was mixed with 40% glycerin in equal volume and stored at -80°C for long-term storage.
[0047] Among the many isolated ...
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