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Pseudomonas mosselii and application thereof

A technology of Pseudomonas morganii and Pseudomonas, which is applied in the field of Pseudomonas, can solve problems such as environmental pollution, pesticide residues, and drug resistance of pathogenic bacteria, and achieve excellent antibacterial effect and good antibacterial effect Effect

Active Publication Date: 2019-01-18
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, chemical pesticides are mainly used to control them, but often due to environmental reasons such as rainfall, the control effect is not good, and it also brings many problems such as environmental pollution, pesticide residues, and pathogenic bacteria resistance.
[0004] At present, the research on the use of antagonistic microorganisms to control plant diseases has made rapid progress, but there are relatively few reports on the use of antagonistic microorganisms to control rice false smut

Method used

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  • Pseudomonas mosselii and application thereof
  • Pseudomonas mosselii and application thereof
  • Pseudomonas mosselii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0044] 1. Isolation and purification of strains

[0045] Five points were randomly selected from the rice planting area of ​​the Fuyang Base of the China National Rice Research Institute to dig out the rice roots. The floating soil on the root system was gently washed with running water. The soil closely attached to the root system was washed off by ultrasonic vibration. Dilute the soil suspension under gradient and apply it to an LB petri dish. Place the petri dish in a constant temperature incubator at 28°C. Whenever a colony grows, pick different morphological colonies on a fresh LB medium plate for streaking purification , And then pick a single colony on the LB solid slope at 4℃ for short-term storage for activity determination.

[0046] The purified single colony was inoculated into LB liquid, and after shaking culture at 28°C overnight, the bacterial liquid was mixed with 40% glycerin in equal volume and stored at -80°C for long-term storage.

[0047] Among the many isolated ...

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PUM

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Abstract

The invention relates to the technical field of pseudomonas, in particular to a Pseudomonas morsselii (Pseudomonas mosselii) bacterium. It is deposited in China Typical Culture Collection, CCTCC NO: M2018534. It has bacteriostatic effect on rice smut, rice blast, bakanae and bacterial leaf blight.

Description

Technical field [0001] The present invention relates to the technical field of Pseudomonas, in particular to a Pseudomonas mosselii (Pseudomonas mosselii) bacteria. Background technique [0002] Fungal diseases seriously endanger global food security, and 70%-80% of plant diseases are caused by pathogenic fungi. Rice blast, sheath blight and bacterial leaf blight are the three main diseases of rice. Rice smut is a fungal disease caused by Ustilaginoidea virens (Ustilaginoidea virens) infecting rice. It occurs from the stage of flowering to milky rice. The disease is widely distributed and occurs in rice areas in Asia, America, Africa and Europe. In addition to reducing rice yields, the toxin (sclerotin) produced by rice smut can affect human and animal safety and farmland ecological environment. Serious hidden dangers. [0003] In recent years, with the accelerated renewal of rice varieties, frequent transfers, increased fertilization levels, and large-scale rice planting, rice ...

Claims

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Application Information

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IPC IPC(8): C12N1/21A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 冯国忠吴丽娟严清陈国庆
Owner CHINA NAT RICE RES INST
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