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Cell-packaged thermo-sensitive hydrogel material with response temperature adjusted as well as preparation method and application thereof

A temperature-sensitive hydrogel and a technology to adjust the response, which is applied in the fields of pharmaceutical formulation, medical science, prosthesis, etc., can solve the problems of complex process and high manufacturing cost, and achieve the effect of simple process, low cost and increased gelation speed

Inactive Publication Date: 2019-01-18
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current cell carriers have high manufacturing costs, complicated procedures, and even a small amount of biological toxicity

Method used

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  • Cell-packaged thermo-sensitive hydrogel material with response temperature adjusted as well as preparation method and application thereof
  • Cell-packaged thermo-sensitive hydrogel material with response temperature adjusted as well as preparation method and application thereof
  • Cell-packaged thermo-sensitive hydrogel material with response temperature adjusted as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Weigh 0.68182g methylcellulose, 0.4g PEG-20000 (80mg / ml), 0.179g Na 2 HPO 3 .12H 2 O (14.2mg / ml), 0.4g xylitol (80mg / ml), dissolved in 5ml 100-degree deionized water, stirred evenly, put into 4-degree refrigerator overnight to completely dissolve. The rheological test (heating speed 0.3℃ / min, strain 0.05% and frequency 1rad / s) obtained its gel temperature at 31.8 degrees.

[0042] Take 1ml of the solution and mix it with the embryonic stem cell suspension, stir evenly on a shaker, and put it into a 37 degree incubator for one hour to form a gel state. The gelled hydrogel is absorbed by the syringe with the needle removed, and the ischemic tissue is injected after the needle is installed.

Embodiment 2

[0044] Weigh 0.68182g methylcellulose, 0.4g PEG-20000 (80mg / ml), 0.179g Na 2 HPO 3 .12H 2O (14.2mg / ml), 0.2g xylitol (40mg / ml), dissolved in 5ml 100-degree deionized water, stirred evenly, put into 4-degree refrigerator overnight to completely dissolve. The rheological test (heating speed 0.3℃ / min, strain 0.05% and frequency 1rad / s) obtained its gel temperature at 33.4 degrees.

[0045] Take 1ml of the solution and mix it with the embryonic stem cell suspension, stir evenly on a shaker, and put it into a 37 degree incubator for one hour to form a gel state. The gelled hydrogel is absorbed by the syringe with the needle removed, and the ischemic tissue is injected after the needle is installed.

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Abstract

The invention belongs to the fields of intelligent materials, high polymer materials and biomedical engineering and specifically relates to a cell-packaged thermo-sensitive hydrogel material with response temperature adjusted as well as a preparation method and application thereof. Cell-packaged thermo-sensitive hydrogel comprises the following raw ingredients by weight percentage: 12wt% of methylcellulose, 7.4wt% of PEG (polyethylene glycol)-20000, 1.4wt% of non-toxic inorganic sodium salt, 0-16wt% of xylitol and the balance of water. The non-toxic inorganic sodium salt is Na2HPO3.12H2O. According to the cell-packaged thermo-sensitive hydrogel material with response temperature adjusted, the thermo-sensitivity response temperature can be adjusted by adjusting the content of the xylitol,and the material has no biotoxicity. When the temperature is low, the material keeps a transparent liquid state; when the temperature is risen to phase inversion temperature, the material can be changed into a gel state. Additionally, the material has no biotoxicity, so that the material can be used for packaging and protecting a cell, and the packaged cell is then injected into a human body.

Description

technical field [0001] The invention belongs to the fields of intelligent materials, polymer materials and biomedical engineering, and specifically relates to a temperature-sensitive hydrogel-encapsulated cell material capable of adjusting response temperature, a preparation method and application thereof. Background technique [0002] Stem cell therapy is a promising method for tissue repair, and various types of stem cells have entered clinical trials. But when transplanting allogeneic or xenogeneic cells by direct injection, their efficacy is limited by the low retention and engraftment of transplanted cells, poor cell retention, and the potential risk of inflammation and immune response. Therefore, it is very important to protect the hydrogel from damage when it is injected into the body as a cell carrier to wrap the cells (van Berlo, J.H.; Molkentin, J.D.An Emerging Consensus on Cardiac Regeneration.Nat.Med.2014,20, 1386-93). [0003] An attractive strategy is to enca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L1/28C08L71/02C08K5/053C08K5/098C08K3/16C08K3/32C08J3/075A61L27/52A61L27/50A61L27/38A61L27/12A61L27/02A61L27/00A61L27/18A61L27/20
CPCA61L27/00A61L27/025A61L27/12A61L27/18A61L27/20A61L27/3834A61L27/50A61L27/52A61L2400/06C08J3/075C08J2301/28C08J2471/02C08K3/16C08K5/053C08K5/098C08K2003/324C08L1/28C08L71/02
Inventor 袁伟忠汪芮谢晓云姚雪亮
Owner TONGJI UNIV
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