Sustained-release system of collagen scaffold loaded with bioactive factors and construction method thereof

Abstract

The invention discloses a sustained-release system of a collagen scaffold loaded with bioactive factors and a construction method thereof. The construction method comprises the following steps: the collagen scaffold is immersed in a solution containing more than one bioactive factor fused with a collagen-specific binding peptide and then washed to remove the bioactive factor not bound to the collagen scaffold; And placing the collagen scaffold into the polymer solution and reacting; The collagen scaffolds were washed, then impregnated in collagen solution, and then washed. The collagen scaffold loaded with bioactive factor has good biocompatibility, It can realize long-term slow and controllable release of bioactive factors, stable and controllable release amount and release rate, and canput an end to sudden release phenomenon. At the same time, it has simple construction method, good controllability, mild reaction conditions, and has great application potential in biological tissue damage repair, slow and controlled release of drugs and other aspects.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to a slow-release system of a collagen scaffold loaded with one or more bioactive factors, its construction method and application. Background technique [0002] The repair of spinal cord injury is still a global medical problem, which brings a heavy burden to individuals, families and society. More and more researchers have proved that the lack of neurotrophic factors at the injury site is one of the important factors hindering injury repair. Therefore, the supplementation of neurochemical factors at the injured site has become one of the breakthroughs in the treatment of spinal cord injury. [0003] At present, the factors that have been found to have neurotrophic effects mainly include brain-derived neurotrophic factor (BDNF), neurotrophic factor NT-3, glial cell-derived neurotrophic factor (GDNF), basic fibroblast growth factor ( bFGF), etc., have the function of protec...

AI Technical Summary

Problems solved by technology

[0005] Most of the traditional sustained-release strategies use microcapsules and microspheres to coat the protein factors and then load them on the scaffold material, relying on the degradation of the wrapping materials to release the protein factors slowly. However, a major defect of this method is that the loaded particles are unstable and easily released from the scaffold. slip off

In addition, some researchers have blended protein factors with gel materials to construct protein factor sustained-release scaffolds. In this method, since protein factors participate in the preparation process of gel scaffolds, they may undergo organic solvents, freeze-drying, and sterilization. Bacteria and other processes are likely to cause loss of activity

In recent years, the method of layer-by-layer self-assembly has been introduced into the slow-release system of drugs. So far, most of the methods use the interaction of hydrogen bonds, van der Waals forces, hydrophobic interactions, covalent bonds, and hydrophilic interactions. However, the combination of these methods is weak or poor in stability, so that the resulting sustained release system still has defects such as difficult to control the drug release rate and release amount stably, for example, "burst release" will occur, resulting in poor therapeutic effect, or even can cause side effects

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