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Preparation method of mulberry leaf alkaloids and application of prepared mulberry leaf alkaloids

A technology of mulberry leaf alkaloids and medicines, applied in the field of alkaloid extraction, can solve the problems of secondary pollution of organic solvents, low alkaloid purity, and low relative content of alkaloids

Active Publication Date: 2019-01-18
SOUTHWEST UNIV
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Problems solved by technology

[0004] At present, the extraction process of traditional Chinese medicine alkaloids is relatively mature. According to the physical and chemical characteristics of alkaloids, suitable solvents such as water, acidic water, acid alcohols, and alkali alcohols are used to extract alkaloids by dipping, percolation, decoction, and enzymatic hydrolysis. However, due to the relatively low content of alkaloids in traditional Chinese medicine, multi-step reactions are required, and a large amount of organic solvents are required. The final alkaloids have low purity and may be secondary polluted by organic solvents

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  • Preparation method of mulberry leaf alkaloids and application of prepared mulberry leaf alkaloids
  • Preparation method of mulberry leaf alkaloids and application of prepared mulberry leaf alkaloids

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[0040] The invention provides a preparation method of mulberry leaf alkaloids, which comprises the following steps: 1) mixing mulberry leaves with an aqueous ethanol solution according to a solid-liquid ratio of 1 g: (15-26) mL to obtain a mixture;

[0041] 2) The mixture obtained in step 1) is ultrasonically filtered to obtain a filtrate; the power of the ultrasonic wave is 720-900W;

[0042] 3) Concentrating the filtrate obtained in step 2) to obtain a concentrate;

[0043] 4) Purify the concentrated solution obtained in step 3) through a column to obtain mulberry leaf alkaloids; the column for column purification includes 732 type cation exchange resin and D101 type macroporous adsorption resin.

[0044] In the preparation method of the mulberry leaf alkaloid of the present invention, the mulberry leaf and the aqueous ethanol solution are mixed according to a solid-liquid ratio of 1 g: (15-26) mL to obtain a mixture. The mulberry leaves in the present invention are prefera...

Embodiment 1

[0061] Weigh the mulberry leaf powder, add it into 60% ethanol aqueous solution according to the ratio of material to liquid 1:20 (g:mL), put it in an ultrasonic cleaning machine with an ultrasonic frequency of 800W for 10min, and vacuum filter (qualitative filter paper, medium speed, Φ9cm ) was filtered to obtain the filtrate, and the combined filtrate was extracted 3 times, evaporated and concentrated to a volume ratio of 2:1 by rotating at 80°C (rotating speed: 100rpm) for later use.

[0062] The obtained concentrated solution was first purified by passing through a D101 macroporous adsorption resin column, and the effluent was collected for future use.

[0063] Add 4 volumes of absolute ethanol to the effluent, and precipitate polysaccharide overnight at 4°C. The filtrate was centrifuged at 4°C, 5000r / min for 10min, the precipitate was discarded to obtain the supernatant, and the supernatant was concentrated by rotary evaporation (rotating at 100rpm) to remove ethanol unti...

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Abstract

The invention provides a preparation method of mulberry leaf alkaloids and application of the prepared mulberry leaf alkaloids, and relates to the technical field of alkaloid extraction. The preparation method comprises the following steps: 1) mixing mulberry leaves and ethanol aqueous solution accord to a material-liquid ratio of 1g: (15-26) mL to obtain a mixture; 2) mixing that mulberry leavesand ethanol aqueous solution according to a material-liquid ratio of (15-26) mL to obtain a mixture; 2) filtering the mixture obtained in the step 1) by ultrasonic wave to obtain filtrate; The power of the ultrasonic wave is 720-900W; 3) concentrating the filtrate obtained in the step 2) to obtain a concentrated solution; 4) purifying that concentrated solution obtain in the step 3) through a column to obtain mulberry leaf alkaloid. The mulberry leaf alkaloid prepared by the method of the invention has a purity of more than 60%, and can be used for preparing medicine for treating liver function damage, improving liver tissue inflammatory reaction and inhibiting liver cell apoptosis.

Description

technical field [0001] The invention belongs to the technical field of extraction of alkaloids, and in particular relates to a preparation method of mulberry leaf alkaloids and an application of the prepared mulberry leaf alkaloids. Background technique [0002] With the development of my country's economy, the intake of fat and cholesterol in residents' diet has increased, and the energy supply ratio of fat has continued to increase, and high-fat diet has become a daily diet. A long-term high-fat diet will not only lead to hyperlipidemia, but also lead to fat accumulation in the liver, liver cell degeneration, and non-alcoholic liver damage. Excessive fat accumulation can develop into fatty liver, which can cause liver damage. Statistics from hospitals at home and abroad show that the number of patients with liver injury is increasing year by year. In response to this trend, many liver-protecting drugs and health foods have appeared on the market. Thousands of these chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P1/16A61K127/00
CPCA61K36/605A61K2236/333A61K2236/55A61P1/16
Inventor 黄先智丁晓雯彭哓蝶沈诗桀
Owner SOUTHWEST UNIV
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